Worth a read

Conventional tissue culture substrates do not accurately emulate in vivo surface conditions. This project involves the development and characterization of a polydimethylsiloxane (PDMS) substrate for cell culture. PDMS is an important biomaterial due to its optical transparency, biocompatibility, and variable elastic modulus. ADSCs and HUVECs were used to analyze spreading and proliferation on PDMS-based substrates of…

As a follow up to my previous post, I would like to present some preliminary data on the subject of endothelial cell alignment under shear stress with variable substrate stiffness. The flow set-up (Figure 1) allows for steady flow across the membrane on which the HUVECs are cultured. No Gel HUVEC Alignment Alignment of HUVECs…

I’ve updated the figure from this post to add new data from oxidized and pre-washed glass samples. We now have possession of teflon material, which I will be testing this week.

Manufacturer’s Data Logged by: Ahmet Gurcan Membrane Type: MSSN 1 micron Microslit – Gold Coated SKU: MSSN400.C-3LX.1-1.0E2-AU Lot Number: 240918-24 Quantity Received: 200 Manufacturer Test Report: Flow Data Avg in SLPM 4psi: 4.5 15psi: 10.58 Burst: 66.78 PSI

Wafer 415-T has been delivered to the lab. It was processed with TEOS and annealed at 950 C. There are a few usable samples, especially along the edge of the wafer. Pinhole density increases as you move towards the center. The uniformity across the wafer is good. From left to right, top to bottom: (0,1)…

Endotoxins (~10-1000kDa) are structural components of bacteria (LPS is the best known) that are released from bacteria when they die.  Endotoxins cause inflammatory responses in humans and animals (infections give you a fever) and in cultured cells.  For most cell culture applications, you don’t have to worry too much about endotoxins.  For monolayer permeability experiments,…