Worth a read

A quick recap of the goals and initial experiments: https://trace-bmps.org/electrospinning-aligned-polymeric-fibers-on-parylene-stensils/ After obtaining a reproducible optimized fiber deposition settings and an improved device design, we moved on to growing HUVECs on these devices as the next step. As previously stated, given that there is a parallel project with membrane masking of ECM with parylene stencils, we…

During my visit last, I deposited a couple of wafers and one of them is to test a even thicker Si film from the NSN stack. The thickness for the Si film is 200nm and the nitride layers are still 30nm. After deposition, the Si wafer was etched and the pop-out TEM grids were then…

Very last minute but here goes … Voting closes at 7 pm tonight. Vote for 2! [yop_poll id=”4″]  

This will be a very brief post, but kind of an interesting one. I have been attempting to get my identification of exosomes by immunogold labeling and that hasn’t been going terribly well. I think that it is due to a wealth of complications including a lack of fixed exosomes, salt contamination and other problems….

The following are TECAN spectrum of the filtrates (and a spectrum of unconjugated peptide) for a series of sequential separations performed on the Barcikowski peptide (CE12W) using 10, 20, and 30nm cutoff Sepcons performed last weekend. Note that all of this data was taken with the quartz cuvette not inserted fully into the TECAN as…

Bob and I are trying to determine which wet etch would be appropriate to use to remove the native oxide inside the pores before membrane liftoff. The issue is when removing the native oxide, we will remove part of the membrane. So far, we removed native oxide from bare silicon wafers using 50:1 HF. For…