As per Chris request, here is a photo with a brief description.
Sorry the image is still not good… the surface of the plastic reflects lights and it is transparent, plus I am using a poor camera. But I hope the description is useful and anyway tomorrow we can check it out in the meeting!
While I have been making nanoporous MgF2 for a while, it has become obvious that the strength of the material is handicapping our ability to generate data; as the membranes rupture we lose weeks of cell culture work. While we have generated some interesting Raman and cellular effects using the nanoporous MgF2 membranes, they are…
Since my last update things have been going quite smoothly. Most pores I make are able to pass 3 or 4 samples before clogging, and I have filled in the various graphs quite nicely. I still would like to flesh out the low and high end of the DNA length spectrum, but I’ve started writing…
In previous experiments where the affinity based capture of EVs in ALine devices was tested, issues were encountered. It was noticed that when capturing EVs on the ALine, the non-biotinylated slit membranes had captured a higher quantity of EVs compared to biotinylated membranes. This conclusion led us to question if the flow through the ALine…
I have uploaded the TEOS process outline: TEOS is tetra-ethyl-ortho-silicate, or equivalently tetra-ethoxy-silane. TEOS slowly hydrolyzes into silicon dioxide and ethanol when in contact with ambient moisture. IMPROVEMENTS: -This process prevents us from having to expose the front-side of the silicon during processing. Concurrently, this eliminates the need for aggressive cleaning of the front surface…
Introduction This post is meant to introduce the new NPN-enabled slide-based devices produced by Ibidi. These devices feature an NPN membrane and microfluidic channels positioned perpendicular to one another formed by PSA and COP layers (Figure 1). Channels above and below the membrane allow for flow-based experiments including tangential flow filtration (TFF) such that biofluids…
We’ve had quite a bit of success transferring nanoporous membranes now, but a lot of questions about the exact nature of the physics at work during the transfer process remain. Pursuant to those questions, we wanted to try changing a variable — switching from nanopores to micropores — to see how that changes the behavior of…