As per Chris request, here is a photo with a brief description.
Sorry the image is still not good… the surface of the plastic reflects lights and it is transparent, plus I am using a poor camera. But I hope the description is useful and anyway tomorrow we can check it out in the meeting!
Introduction Breakdown of the blood-brain barrier (BBB) is one of the earliest signs of sepsis and is linked with long-term cognitive impairment. As animal models poorly recapitulate human sepsis, alternative approaches are direly needed to understand sepsis-associated brain injury and develop strategies that protect against cognitive decline. To fill this gap, we are developing a…
After reading and discussing a paper in journal club that talked about cells having a “mechanical memory” (found here “Mechanical memory and dosing influence stem cell fate“), we decided to replicate our previous YAP/TAZ experiments with passage one HUVECs direct from Life Technologies as to avoid making our cells “remember” that they had ever been plated…
Recently, I have been exploring the temperature dependence of post-Si deposition Ar sputtered films (15 nm thick). Below is a comparison of the average pore size, porosity, and density of pores of Ar and non-Ar sputtered films annealed between 800 C and 1100 C. It appears that Ar sputtering increases the avg. diameter and porosity…
Dave deposited a series of wafers last week at different temperatures. SC 166 -SC 173 were deposited between RT and 300C. These wafers had Jess’ E.O mask pattern ( circular sepcon) with single , 3,6 and 9 squares. The wafers were mostly pinhole free.In order to determine if deposition temperature affected the nature/ strength of…
Two simple diffusion separations were performed before break using membranes from wafers 205 and 213 (locations unknown as these were salvaged/misplaced chips). I ran the samples I collected on a 10% SDS-PAGE gel today. It seems that the membranes were able to separate the standards at two different cutoffs. The missing band in the middle…
In my last post, I concluded that switching the o-ring position from below the membrane to the top in the sepcon produced no leaks. The reason for this switch was to reduce the 1mm “dead-space” (created by the o-ring) that adds to the unstirred water layer (UWL). The next thing that Barrett and I looked…