Coulter Project Timeline

Hello Everyone, Following my experiments with Track Etched Membranes: So we came up with this idea to perform SEM imaging. Following are the images that i took for my experiment with 100 nm beads over 100 nm pores after releasing step: So in this image, we can see that there are a lot of beads…

Goal: study HUVEC migration on different porous substrates. HP = high porosity 0.5 μm SiO2 membrane LP = low porosity 0.5 μm SiO2 membrane NP = non-porous SiO2 membrane TCP = tissue culture polystyrene (~3 GPa [ref 1, 2]) P184 = sylgard 184 (a commonly used PDMS; Young’s modulus = 1.74-1.84 MPa [ref 3]) P527 = sylgard 527…

To further investigate the mechanism of discoloration, I prepared buffers at different pH’s and measured discoloration and pH over time. Buffers were: 0.1M MES, pH=pKa=6.1; 0.1M Tris-Base, pH=pKa=8.39; 0.1M Tris-HCl, pH=pKa=8.35; 10mM HEPES in 1X HBSS, pH 7.38, where HEPES pKa = 7.31. This was done in the incubator. Experiment 1: Experiment 2: pH vs….

I recently popped out membranes from wafers 1064 and 1065 for our lab. I only started with about half a wafer, but these are the numbers from the extraction: Wafer ID # of In-Tact Extracted Membranes : High/Low Porosity # I broke during extraction # already broken before extraction # apparently under etched 1065 (100W)…

Hello Everyone, Following of making figures for our first exosome paper, I needed to have SEM images after releasing step. So i ran another experiment and checked the membrane after releasing step. You can see the SEM images of this step:   As you’ve probably noticed, compared to after capturing step, you see less captured…