Purchasing 101

We had previously tested different surfactants to see if we could get 20 nm polystyrene beads and IgG to stay in suspension while filtering with the centrifuge.  We found 0.1% Tween 20 to be sufficient when dealing with 10:1 (5×10^14) 20 nm NPs and 1mg/ml IgG. During the reverse centrifuge studies we mixed 10^15 particles/ml…

UPDATE: Figure normalized by frontal surface area. This brings the glass data in line with pnc-Si (which is similar to my more recent fluorescence data). Here is my first try at putting together all the recent adsorption gels I’ve done. This data has all been obtained using the more vigorous washing step. All densitometry performed…

20240515 MPS 2024 poster

As a proof of principal experiment, we wanted to test if Kilean and I could extract RNA from exosomes following their capture on 1298 membranes.  As noted in Figure 1, we first used ultrafiltration to capture exosomes on the nanoporous membrane.  We then attempted exosome lysis and RNA release by  flowing the lysate below the…

Chris and I visited the AJA sputter company in Boston last week.  The tool was fully functional and we were able to deposit several test wafers.  Overall, we were impressed with the performance.  The uniformity across a 6″ substrate was sub-1 %.  This is certainly a huge improvement over the 3-5% uniformity we currently achieve…

This is kind of a shot in the dark, but I wanted to see if I could get a zeta potential for pnc-Si that was as high as predicted by the electroosmosis results. First I tried to get the membranes to break in 1mL of PBS so that the windows were floating on top of…