Purchasing 101

I ordered some new AC160 tips last week from Asylum and got on the AFM Thursday to test them out.  I think they solved some of the problems I was running into over the last few weeks.  Mainly, I was finally able to image entirely in repulsive mode (which was impossible with the older tips).  All of…

Carrie and I have been trying to detect HMM (APOBEC3G) diffusion through Sepcons without much luck.  We determined that the dilution factor (~1:30) for diffusion in our 384-well plate format was too high to detect HMM in the basolateral well.  To try to get around this problem, I decreased the volume (and thus dilution factor)…

Wafer 398 was deposited on 2/14/2008 but just etched yesterday in the new cell (it’s a beauty esp. when you compare it to the old one). I’ve etched several wafers now in the new cell and none of the membranes show signs of cigarette burns or tears. Good news in regards to the copper contamination…

In most of the images I’ve posted of bEnd3 on pnc-Si, it seemed like there were more cells crowded onto the free-standing pnc-Si compared to supported pnc-Si.  This post shows that this is indeed true. To do cell counts, I stained cells with Hoechst 33342 (1uM in media) for 15 minutes @ room temperature and…