Modular assembly of the µSiM


Design and Manufacturing Plans from Aline: 2019-09-23 Rochester Assembly Step by Step
Prototype Runs @ ALine



Post-fabrication assembly (cutting with lower power to eliminate bubbles) …





Design and Manufacturing Plans from Aline: 2019-09-23 Rochester Assembly Step by Step
Prototype Runs @ ALine



Post-fabrication assembly (cutting with lower power to eliminate bubbles) …



In this experiment, I wanted to test how quickly vacuoles form in endothelial cells when cultured on pnc-Si transwells. Here I used 30nm SC502 RTP’ed samples with the pnc-Si side down. I seeded P9 bEnd3 cells at 50000 cells/cm2 on the pnc-Si side, allowed the cells to attach for 2 hours and then inverted the…
For many months, Emma and I are trying to achieve neutrophil migration through endothelial cells and through the underlying collagen gel. I need to explain the details of my device first. The schematic is shown as below. I am using a 3 um porous membrane to allow neutrophils to cross the HUVECs in the collagen…
A quick recap: of the pores using the old NPN batch, 2 of them showed folding suppression. The first one I was able to collect events for 1000 and 2000 bp dsDNA, bnoth of which showed almost no folding, but had an unusually low event rate. The second suppressed folding for the first half of…
I submitted a proposal last week with Tom & I as co-PI’s to NSF’s Process Reaction Engineering division in CBET. Novel Catalytic Membrane Reactor Based on Ultra-thin Palladium Film Supported on Nano-structured Inorganic Membrane I’m attaching the intro. paragraph and a couple of the figures to give a general idea of what the proposal is…
There are four main challenges for making such a thin photoresist gasket on a little chip. Q1: How can I get under 1um thickness photoresist gasket? Q2: Is the adhesion of photoresist gasket strong enough for further usage? Q3: How can I write the pattern onto the gasket that will have the center window open?…
In my previous post, I derived a formula for the time-dependent flux decline in a centrifuge-driven membrane separation, assuming that the resistance of the membrane was held constant. The problem with that approach is that, as Josh discusses in this post, the resistance of the membrane increases with time according to one of several standard…