Modular assembly of the µSiM

This update covers my efforts since our last skype meeting to show DNA size separation in a filter, as well as some very interesting TEM images of ALD-coated NPN and associated controls. Here is an inventory of my current chips: Oxide-spacer chips with nitride cap and no filter yet: 8 Oxide-spacer chips with nitride cap…

The purpose of this device is to make uniform and straight scratch wounds in a 96 and 24 well plate for migration experiments. Previously, scratch wounds were made by hand using a p-10 pipette tip by scraping the tip in a straight line along the bottom of the well. Not only is manually scratching the…

We manufactured a wafer specifically for SERS experiments in the hope of recapturing larger pore distributions/pore densities that we observed in some of the prototype runs of NPN. Natively, it’s a little thicker, and a little less porous than the 1085 material. By partially etching the membranes from the backside, we should be able to…

I set out to do an in depth study of adsorption to various surfaces using the sample buffer method. Midway through staining the multiple gels I noticed there was a problem. It looked as though glass and polystyrene adsorbed about the same amount of BSA as did pnc-Si. Details of the setup follow. Preparation: Using…

For last many months, I am working on neutrophil migration experiments to develop protocols to inhibit them from traversing the basement membrane. My previous blogs are here and here. I want to prevent neutrophils from crossing the endothelial basement membrane (BM), and record the changes in TEER and try to correlate with the gain in…