TEM images of actin filaments on Formvar grid

Using methods pretty much identical to those outlined in Tuesday’s post, I was able to get very similar data for a different BSA concentration. Whereas Tuesday’s curves were all done with 1mg/mL BSA, this was done with 0.5mg/mL BSA. The first time I collected sample from the 5 slot chip during the buffer separations, I let…

We’ve had quite a bit of success transferring nanoporous membranes now, but a lot of questions about the exact nature of the physics at work during the transfer process remain. Pursuant to those questions, we wanted to try changing a variable — switching from nanopores to micropores — to see how that changes the behavior of…

I am trying to deposit metal on the membrane with minimal deposition inside the pores. The image below is an example of how the film quality and thickness change when the sample is positioned at different angles and distances from the source in the same deposition run. The schematic in the corner of each SEM…

I attempted a diffusion study with w685 three days ago. If you were following the comments on the w685 page, you’ll know I had some difficulty pipetting the protein solution into the well. I haven’t had this problem recently, and when I used to encounter this problem I would etch the remaining backside oxide with…