Porous TEM windows fabrication using CsCl self-assembly
The competition keeps coming at us …
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New Cartridge from INT
INT received the first samples of their new cartridge design. The following slides were given to us by INT to provide some detail. The shorter cylinder on the left that is called the “glass bead pod” needs on filter fixed to the bottom. The taller cylinder is the “desalting chamber” and will require a filter…
Effect of Membrane Morphology on System Capacity During Normal Flow Microfiltration (review)
pdf of Zydney Paper This paper tackles the concept of membrane capacity – the amount of fluid a membrane can pass before it’s flux declines (due to pore blockage or the buildup of a cake) to some arbitrary fraction of the initial flux. Common practice in industry (according to the paper) is to calculate capacity…
Preliminary potentiostat measurements on iBidi format devices
Thanks to Sidahmed Abayzeed and Emilia Moradi. Summary: I’ve started making impedance spectroscopy measurements with a potentiostat in the iBidi devices. The data is noisy, but there is a clear change in the curve characteristic as cells grow upon the nanomembrane. NPN seems to be working better than MgF2, for these measurements. I’ve measured the impedance…
Detection Limit for Sodium Fluorescein
We now have a linear standard curve for sodium fluorescein (Na-F) in clear 24-well plates. What is the detection limit? The detection limit is defined as the lowest background-corrected concentration that is 3 standard deviations above the noise of the background. The background here is HBSS with 10mM HEPES. I measured the dilutions a four…
Inventory of Nanoparticles
Nanoparticles Inventory – taken by Karl on 11/2/12. All these particles can be found in the refrigerator. Manufacturer size type amount (approx) expiration date fluorescence (if applicable) BBI 5nm gold colloid 3mL Jan-10 BBI 5nm gold colloid 6mL Aug-11 BBI 10nm gold colloid 2mL Jun-11 BBI 10nm gold colloid 2mL May-09 BBI 15nm gold colloid…
Burst Pressure, Permeance of NSN pnc-Si SEPCONS with large pores
Karl, Charles and I measured the burst pressure and permeance of some 30-50-30 and 30-100-30 NSN. Burst Pressure: The SEPCONs readily burst at pressures under 1 psi when the flow was directed from the backside of the chip through the membrane. The membranes were able to withstand higher pressures when the pressure was applied from…