Viscosity vs. [BSA]
Are you wondering why Dean has yet to go beyond 30% BSA in his clearance studies? Here is why …
APP-04_Viscosity-of-BSA-in-PBS.pdfSimilar Posts
Proposed Dielectrophoresis (DEP) Tests w/ Microporous SiOx Membranes
The Gaborski lab has been working with Blanca’s lab performing some scoping tests w/ SiN and SiOx microporous membranes. The aim is obtain proof of concept results regarding the efficacy of using a microporous membrane to trap sub-micron particles with DEP. Last week, we tested w/ our 200 nm polycarbonate beads. We were able to…
Using UV Ozone to Bond PDMS to Glass
ByMike HI talked with Jim last Friday about a second possible dialysis system using PDMS bound to a glass cover slip. I made a batch of PDMS and let it cure at room temperature over the past few days. Jim had suggested I try bonding the PDMS to a glass cover slip using the UV/Ozone system…
Wafer Testing: W520, 558, 559
I recently received three pinhole-ridden wafers for cell-related experiments. All three have been RTPed at 800C for 5 minutes in argon, which is the typical procedure we have been using for cell culture. The bursts pressures for each wafer are shown below. W520 is weaker than 558 and 559; I plan on using the stronger…
HUVEC Growth on Various Substrates
In evaluating the usefulness of our membranes for cell culture, it is important to understand if and how cell growth is affected by the surface. The desire is that they be consistent with standard culture substrates (i.e. Tissue Culture Plastic (TC)). I performed two growth trials using HUVECs (Human Umbilical Vein Endothelial Cells), one focusing…
Tangential Flow Filtration of Gold Nanoparticles: A Model Exosome System
ByKileanThis is the second in my series of posts that will be on my model exosome system. As I am trying to define the appropriate conditions for separating exosomes from blood and other fluids, I am first going through and trying to isolate each important parameter and see how they affect the capture of these…
hiPSC Differentiation to Endothelial Cell Culture Method, Brain Microvascular Endothelial Cells (EECM-BMEC-like cells), Round 2
Introduction This post highlights results from my second attempt to differentiate hiPSCs into endothelial progenitor cells EECM-BMEC-like cells in our lab. For details on methodology, see Nishihara et al., FASEB, 2020 and my previous post. Methods Cells were seeded at 70,000 and 100,000 cells/well on D-3. Only the 100,000 cell/well plate was sorted. Due to media being backordered,…