Inspired by JP’s work with the Veeco Wyko, I attempted to fit pressure-displacement curves with the equation for thin film deformation (Vlassak and Nix 1992) for two membrane geometries: 100um x 100um and 1000um x 1000um. The relationship between displacement and pressure is: P = pressure, d = displacement, C1 and C2 are geometric coefficients,…
Hi all, During last NRG meeting some questions are raised on what is the transmembrane pressure in Dean’s chip-in-a-beaker dialysis system and what is the direction of flow of the whole system. i gave them some thought and proposed the following scenario. Please feel free to correct me should I made any mistake. i am simply throwing…
As discussed in a previous post, we decided to test chips from wafer #1070 because they have smaller pores than those used in the most recent forward vs. reverse study. In the forward direction, 31 uL of filtrate was passed after 30 minutes at 3 krpm. (after 30 additional minutes at 5 krpm the total…
The plan was to fabricate ~7nm pores on the PDMS-painted 200nm SiO2 + 20nm SiN chips and take IVs overtime, and observe the stability of the nanopore’s size. I fabricated a total of 8 200nm SiO2+20nm SiN chips using the Monolith protocol, to 7 nm. Half of the chips were wetted with the freezer step…
This post is a continuation of project found in Fabricating 0.5 um Channels in Microscope Slides for Staph Invasion Assays. Just to review, after the development of μSiM-CA (Canalicular Array; see Masters et al., Nanomedicine: Nanotechnology, Biology, and Medicine 2019), Jim wanted to develop a platform to answer some more biophysics focused questions. In order…
With the help of Henry and Greg, the Fisher team has been busy learning the basics. John and Ryan have learned some PDMS microfluidics-making and will have their first chips ready on Tuesday. We have also been playing with the silhouette printer, studying our options for producing some simple devices. Finally, we produced some chip designs to…
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It’s not just that dimers can survive the SDS, it’s that there’s “an equilibrium between monomeric and dimeric cyt c in the presence of SDS” – this is shown in figure S1 curve F and G. Adding SDS to purely monomeric solutions makes dimers, adding it to purely dimeric solutions makes monomers. This means we can’t clean up the figures by simply starting with a more pure solution of monomeric cyt c.