Viscosity vs. [BSA]
Are you wondering why Dean has yet to go beyond 30% BSA in his clearance studies? Here is why …
APP-04_Viscosity-of-BSA-in-PBS.pdfSimilar Posts
Protein Adsorption on silanized NPN Chip
ByHenryWe are working with the Shestopalov group to silanize the nanoporous silicon nitride (NPN) membrane chip. In an ideal scenario, the silane would then be conjugated to PEG to ward off protein adsorption. In the presented work, we study protein adsorption on both the untreated and the silanized NPN chips using epifluorescence as the imaging modality….
NP separations with NPN using constant pressure setup
Our recent work using NPN to separate free IgG from 20 nm NPs has shown that we can pass liquid under either forward or reverse configurations using the centrifuge. We had thought that there would be greater resistance in the forward orientation due to cake layer formation. We had found high resistance to flow in…
Production from week 5.5.2008
Last week’s production yielded two wafers (502 & 504) with defect-free membranes. 506 had a high pinhole density. These wafers have a TEOS mask and were annealed twice; the first anneals did not produce pores so they were annealed again at 1000 C. From these results, it seems that the first anneal “seeds” pore formation…
Permeability of NPN vs. Cellulose Triacetate
UPDATE: Some data presented here is out of date. For up-to-date data, see my next post here. — Lately I’ve been performing comparison experiments between our NPN membranes (wafer 1132, 3 slot SEPCON, 40-48nm pores and 13-20% porosity) and commercial cellulose triacetate filtration membranes, with respect to the degree to which they hinder the diffusion…
Streaming Potential Device
This device was built similar to the device featured in the Burns(Zydney) 2000 J. Mem. Sci. paper. Our device seals in a SepCon formatted pnc-Si chip and allows 2 electrodes to be positioned by the chip. A feed line pressurizes the chip with saline, and the generated potential can be measured using the electrodes. The…
PEG-NPN for Reduced Protein Binding
ByJimHopefully in the next week we will be submitting the following manuscript … X. Li, D. Johnson, W. Ma, H. Chung, J. Getpreecharsawas, J.L. McGrath, A.A. Shestopalov, Modification of Nanoporous Silicon Nitride with Stable and Functional Organic Monolayers, ACS Chemistry of Materials (2016) submitted. Here are the highlights … Traditional silanization of silicon materials uses…