Multi channel, dual chip dialysis device

Tweaking simulation was not bringing it any closer to the experimental shown in the last post.  This led me to try the experimental once more.  I set up the same system and used 1:2 dilution of the standard protein mixture.  I then homogenized the retentate and filtrate and ran 10 ul of each on a…

Optimization of the PLL-g-PEG coating on SiO2 substrate: The first goal was developing an efficient method for PLL-g-PEG coating which provides a high density of PLL-g-PEG grafting on SiO2 substrate and effectively prevent cell adhesion. To achieve this goal, different concentrations of PLL-g-PEG in 10mM HEPES solution ranging from 0.1 to 0.9 mg/ml (0.1, 0.3,…

In my last post, I described a new process for reducing fluoropolymer build up during RIE. Unfortunately, the new process had substantially lower yields (half) than the process I was using before. In this post, I am detailing my attempts to make the MgF2 nanomembranes stronger to try and improve the yield of my current etching…

Here are some more images from my ongoing cell culture project. My previous work and protocols: ZO-1 staining of confluent RPE cells on various MgF2 substrates I added twice the amount of primary antibody on these devices that I usually do (1:100 dilution, instead of 1:200).     High Porosity MgF2   TCP MgF2 Coverglass  

One of the things that I’ve been working on recently is trying to explain the extreme elasticity of the pnc-Si material which is connected to its nanocrystalline structure. There are two parts in this question. First, how are amorphous or crystalline phases involved during stretching? Second, does the crystalline phase change under application of mechanical…