Process Flow Update

Hi all, my name is John Cognetti, and I am a first-year PhD student in BME. I recently finished a rotation in the McGrath Lab. My goal was to measure changes in resistance across the membrane as the membrane becomes saturated with particles. Initially, the plan was to use Tejas’ trans-endothelial electrical resistance (TEER) setup…

Last week, JP deposited 25nm SiO2 for me on wafer 644 (which I cleaned in the UV/Ozone before I gave it to him). Unfortunately, someone broke the E-Beam that day and JP was only able to deposit on 1 side of the wafer (the solid side). I did some experiments on the wafer anyway. First,…

The aim of this work was to electrospin aligned polymeric fibers on parylene stencils as means to characterize the critical necessary total area and necessary opening sizes to elicit endothelial cell response in the form of cell alignment among other characteristics typically found in human cells that are adjacent to highly aligned ECM. For this…

This is an update of the research on the on-chip EO pump. Due to a mistake in determining the direction of particle flow, it now looks like the particles in this post were moving by electrophoresis rather than electroosmosis.  The particles were negatively charged (-40 mV zeta potential) because of a COOH group on the…

The movable cutoff gels were more or less successful, but were too dirty for publication (see knowledge page). I’ve been trying to rerun them so that they look nicer, but the darker stain that I used has brought out a few new observations. The first gel following this paragraph is the new gel. The second…

My streaming potential device is now working consistently. It turns out that the problem was the concentrations of salt that I was using. The Debye Length (the distance over which charge-charge interactions are important in an electrolyte) is given as , where   is the debye length in nm and  is the molar concentration of…