Process Flow Update

Yesterday, Chris, Dave, Graham and I were (exhaustively) trained on the Asylum AFM in Rick Waugh’s lab.  We had an overview of the basic features of the AFM and got some real data, as well. The top panel is a height image of the pnc-Si chip with the well side down.  Notice the slight decrease…

During my previous derivations of electroosmotic theory, I described the electroosmotic velocity using the classical Helmholtz-Smoluchowski equation: where ε is the permittivity, E the electric field, ζ the zeta potential, and η the viscosity.  This equations basically says the velocity of electroosmosis is dependent on the electric field across the material and the zeta potential….

In this gel I evaluated the linear range of coomassie brilliant blue. From this test it looks like things are linear from .0156 to .125mg/mL.  My guess is that the protein ladder bands in the previous experiment fall within the range. I used the Bradford assay to find the total concentration of the stock protein…

What happens to cells when they are exposed to a series of bubbles with and without a dubbler? The purpose of this experiment was to prove the hypothesis that bubbles damage cells, and the dubbler prevents cells from experiencing bubble damage. The experiment was performed with a PBS flow rate of about 0.14 mL/min through…

All electrokinetic phenomena are connected by a form of the Helmholtz-Smoluchowski equation.  In the case of electroosmosis, the equation can be written as follows: where v is the velocity (in m/s) of water flow through the pores, epsilon the permittivity, E the electric field, zeta the zeta potential, and eta the viscosity.  Since we do…