New metal coated wafer#502

Introduction The on going work for the dialysis project at UR has undergone changes and many hours of trouble shooting over the past semester. We are at a point now that I would like to share all the work I have done up until this point including assay development, method progression, and the experiments we…

As a follow up to my previous post, I would like to present some preliminary data on the subject of endothelial cell alignment under shear stress with variable substrate stiffness. The flow set-up (Figure 1) allows for steady flow across the membrane on which the HUVECs are cultured. No Gel HUVEC Alignment Alignment of HUVECs…

I recently set up 24 hour diffusion separations with the BioRad protein standards. The following gel shows the separations using w415 position (-4,3) and w416 (-1,3). Different cutoffs were observed with the two membranes.

In my previous transport post here, I showed O-ring placement data which was motivated by my thinking about the unstirred water layer (UWL).  I think it’s helpful to think about the total resistance to transport through transwell membranes as a number of resistors in series.  There is an UWL in the donor well, then the…

Hi all – I finished my 10 months internship with Sartorius on 16th of July and since all my results are going to be the third and last aim of my Ph. D., I will be presenting my results in our NRG meeting. Since these results are confidential, I am not allowed to publish them…