New metal coated wafer#502

Microdialysis: Separation of BSA and Cyto C For the past few months I have been studying how well the 75 nm NPN-O membranes are at clearing cyto c while retaining BSA with and without the presence of PEG coating. Additionally, conductivity tests were performed to determine how well the membrane cleared a small molecule. See…

I continued to run some of the samples that I described in this post last week.  These are P8 bEnd3 cells, seeded at 100000 cells/cm2 (just below confluence) and grown for 14 DAYS.  The pnc-Si was SC126 that was RTP’ed.  I measured the TEER of these samples for 2 weeks and then stained the cells…

Last week we attempted to mimic our standard CVC recipe in the AJA system (15 nm Si, 450 C deposition temp, 1000 C RTP).  I should note that the protective oxide thickness in the AJA membrane was 20 nm (not 40 nm as it was in the CVC).  Below are two images comparing the AJA…

All electrokinetic phenomena are connected by a form of the Helmholtz-Smoluchowski equation.  In the case of electroosmosis, the equation can be written as follows: where v is the velocity (in m/s) of water flow through the pores, epsilon the permittivity, E the electric field, zeta the zeta potential, and eta the viscosity.  Since we do…

Last week, I repeated Anant’s experiment which aimed to eliminate edge effects by painting over chips with nailpolish. I painted the entire well side of the chip but tried to keep the wells clear of polish (“painted back”) and I covered just the edge of a chip but tried to keep polish off the well…