Our new diffusion chamber is finally here. Take a look at the photos first – in our next group meeting you can see the real thing. Today I will test its usability and reliability. Please keep in mind this is only the prototype, there must be upside space for improvement.
Introduction Currently, no FDA approved drug exist for the treatment of sepsis. Past sepsis pharmaceuticals attempted to target mediators of the innate immune response. These drugs, however, struggled to balance remedial immune suppression from complete immune knockdown in a patient population plagued my a maladative immune system. This fundamental issues is due in part to…
A little while back I posted a design for a capture device that would (nominally) work for dialysis on real patients. Due to strict requirements to avoid clotting, the design included a lot of curves and nuanced structures that would be no problem for a lithographical or molding method, but which are a step too…
I’ve tested several membrane types with the streaming potential apparatus in order to calculate zeta potential. Here are the results: All materials in the above chart have negative zeta potentials. Pnc-Si seems to have the highest overall potential, followed by PES then cellulose. Cellulose is prone to cracking and bursting at pressure above 7 PSI….
Hi all, So this is slightly different from my previous post, but is perhaps much more important for the work that I am doing. This analysis is important because it allows us to determine the appropriate conditions at which to operate the system. Currently, I operate the device at a flow rate of 10 microliters/minute…
I wanted to do some more fluroescent imaging in transwells to look into background fluroescence. This was mainly motivated by my inital study. These are HUVECs stained with 5uM CMFDA and 10uM Hoechst 33342, fixed in formaldehyde and imaged on the Zeiss at 10X. This was 6 hours after seeding. Before acquiring the images, I…
I took a closer look at some of Lingyun’s samples today, and captured some interesting TEM images. wafer 205 with 10 nm Ti @ 35kx wafer 205 with 10nm Ti @ 250kx wafer 205 with 10nm Ti & 10nm Au @ 35kx wafer 205 with 10nm Ti & 10nm Au @ 200kx The higher mag….