Synergistic Control of Mesenchymal Stem Cell Differentiation by Nanoscale Surface Geometry and Immobilized Growth Factors on TiO2 Nanotubes

I’m trying to find the best method to stain proteins on nitrocellulose, so we can easily see the protein on the NC membrane. I first tried Coomassie Blue which did not work very well with the NC. The Coomassie was too dark, as seen by the image below. Harold Smith gave me some Ponceau S…

Introduction While we have optimized coculture models for commercial brain endothelial cells and pericytes, we needed to do the same for iPSC-derived cocultures. In this post, I show the optimization process using IMR90-4-derived EECM-BMEC-like cells [1] and brain pericyte-like cells (BPLC) [2]. Methods The top well chip surface was coated with collagen iv (Sigma C5533), bovine fibronectin…

We’ve been focusing on bicarbonate ions as one of the causes of discoloration. So, I made several sodium bicarbonate solutions and tracked discoloration of non-RTP’ed samples. I made 3.7 mg/mL NaHCO3 (=44mM, this is the concentration in DMEM), 1.85 mg/mL NaHCO3, 0.925 mg/mL NaHCO3, 0.4625 mg/mL NaHCO3 and 0.23125 mg/mL NaHCO3. I also ordered a…

I’ve suspected that the barrier function of endothelial cells might be surface-dependent (that is, cells grown on the pnc-Si side vs. cells grown on the well side).  I hypothesized that the interface between membrane and well walls would alter the continuity of the endothelial monolayer when cells are grown on the well wide.  To test…

So, I have been working with a 4-probe TEER measurement system to record the changes in endothelial permeability during neutrophil extravasation and other biochemical assays such as thrombin treatment and TNF stimulation. 4-probe systems are sensitive because they don’t suffer from the contact impedances and measure only the potential drop across the sample under consideration. See…