Flipped sample discoloration in Sepcon

Hello All, I wished to be writing this from some far away tropical resort, as I was told PhD students of the past used to do before they defended their theses. Instead, this comes straight from the ROC homeland, deep in the midst of a global pandemic and a tea-fueled thesis writing session filled with…

I used the transwell track etched membranes for these permeability experiments.  I first looked at 5nm gold in 10mM KCl.  After 24 hours the retentate and filtrate had just about equilibrated: We can compare these scans to the stock 5nm stock solution and see that the height of the 525nm peak is related to the…

I’ve recently been exploring hydraulic permeability using the pressure cell.  As you may recall, the majority of our permeability measurements have been done in the centrifuge (EQ format or closed bucket).  The pressure cell allows for a more accurate measurement of permeability because it is not affected by changing water levels or variable driving pressures….

  Continuing the studies on neutrophil transmigration in devices with embedded parylene membranes of different pore sizes and thicknesses, three pore sizes (1.0 um, 1.4 um and 1.7 um) and two thicknesses (0.7 um and 1.2 um) were used for these sets of experiments. Thickness ratio of 1.2 um to 0.7 um roughly correlated with…

A couple weeks ago when I presented at lab meeting, Angela Glading suggested that I look at epithelial cell vacuolization on transwells.  This was in order to determine if vacuole formation was endothelial cell-specific.  If I’m remembering correctly, the rationale was that the vacuole-less fibroblasts I showed were not contact-inhibited cells and thus not a…