Journal Club for June 26

I’ve been trying to get multi-color images of endothelial cells and glial cells on transwell membranes in order to compare image quality on glass, pnc-Si and polymer membranes.  Specifically, I’ve tried staining endothelial cells green with VEGF mAbs and glial cells red with neurofulament H (NFH) mAbs but VEGF expression in bEnd3 cells is extremely…

Although the connection of those electrical circuits was always unstable, Greg and I did get some TEER measurement with this set up:   Device A Day 1 Day 2 Day 3 Day 4 Day 5 EVOM 1 -3450Ω* -1000Ω -1200 Ω -1000 Ω / EVOM2 100 Ω -9600 Ω 450 Ω -3 Ω 827Ω Device…

After much anticipation, we have confirmed that pores do form with the AJA deposition system!  Below are a series of images of membranes of two different thicknesses that were annealed at several temperatures in the RTP. 1000 C RTP; 40/9/40 stack Amorphous; 40/9/40 stack 1000 C; 40/15/40 stack 800 C; 40/15/40 stack Some immediate observations:…

Cell Culture on the CO2 exchange Devices  The system becomes more complicated with the addition of cells. Tejas Khire cultured HUVECS in a device that had an inverted design to the one pictured above. (The cells were plated on the top portion (well side) of the nanomembrane with media and the bottom (flat side)of the membrane was…

  These Adarza chips were pre wetted with PBS then incubated with fetal bovine serum (FBS) overnight in the refrigerator then rinsed. TRITC (2 mg/mL) added to  chips and incubated at room temperature for one hour. Then rinsed once with reaction buffer then rinsed thoroughly with distilled de-ionized (DDI) water. I first measured using the…

There’s been some discussion on whether furnace-annealed-only wafers are more uniform than RTP’ed ones. Today, I looked at several samples from wafer 304 (FA 1000 C) at different positions along the y-axis. (0, 5) – 25kx (0,2) – 25kx (0,1) – 50kx (0, -1) – 25kx (0, -4) – 25kx There’s a clear change in…