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Adsorption Quantification I
UPDATE: Figure normalized by frontal surface area. This brings the glass data in line with pnc-Si (which is similar to my more recent fluorescence data). Here is my first try at putting together all the recent adsorption gels I’ve done. This data has all been obtained using the more vigorous washing step. All densitometry performed…
Better SEM images of 75 nm membranes
All of the images are from the same 75 nm NPN four-channel chip. The first images shows the edges from the backside of the chip, showing how flat it is (without the oxynitride present on the top side). There is a crackle-like texture but the third image, which shows the corner of the membrane and…
Fluorescence Adsorption Assays
Glass chips and pnc-Si membranes were incubated in 1mg/mL FITC labeled BSA for 30min. They were vigorously washed in a large beaker of PBS. Chips were placed on a slide and imaged with fluorescence scope. For this study, it seems that the fluid surrounding the chip was much brighter than the chip itself. The following…
CRePE-ssNP UR Update Nov. 2021
ByJimTo keep the NRG up-to-date on the goings-on in our collaboration with the University of Ottawa, we will plan to share our progress slide decks from the CRePE-ssNP group meetings that happen every two months. Periodically, we’ll also update the NRG on overall progress of the project including the work done in the two Ottawa…
Zeta Potential Measurements of Microbeads
I tested all the microbeads that the Gaborski and Lapizco-Encinas labs own. The results are summarized below. The sizes ranged from 50 nm to 2 µm. I mixed them to concentrations of 0.2 g/L for all bead less than 1 µm and 1.0 g/L for all 1 µm or greater. Interestingly, the scans look rather…
Permeability Graphs for BSA Separations with Variable-Active-Area Sepcons Show Flow is Independent of Fractional Active Area
What I had in my two previous posts described as permeability graphs were in fact total volume passed/ total time passed graphs: What I thought I saw was that cake layer formation was dependent on the filter geometry. There is some justification for such a dependence in the literature – These folks found that spacer…