Similar Posts
Reducing Fluoropolymer Buildup on MgF2 RIE Process
I have a preliminary result from Kevin that there is a layer of fluoropolymer remaining on my MgF2 freestanding nanomembranes (which could explain some of the carbon signal in EDX from my previous posts). This would create another unnecessary signal for the intended Raman spectroscopy application. Remembering an old graph from my schooling, I dropped…
Upping the ante
I guess we weren’t sure if I was actually drawing rhodamine back through the membrane or just bleaching it. So I made a new apparatus which consisted of a sawed off sepcon mounted on a slide with channels etched into the lower plastic of the device to connect a pbs resevoir on the slide to…
HUVEC Cadherin Immunofluorescence (1)
One of the markers of endothelial monolayer maturation/barrier function is the expression of adherens junction and tight junction proteins. The adherens junction is composed of transmembrane proteins called cadherins (among other proteins) that link cadherins of neighboring cells to the actin cytoskeleton. They are found in most (all?) vertebrates and are necessary for cell-cell adhesion…
Time after RTP – Discoloration
Last week in lab meeting, someone wondered if there the time after RTP affects the rate of discoloration. To test this, I RTP’ed chips from w638 in Ar at 800C for 5 minutes. I then followed discoloration in DMEM+10%FBS in the incubator with untreated w638 chips as controls. All of the chips discolored within a…
First Thoughts on ECMO
In preparation for the ICNMM conference next summer (and more immediately, the paper they’ve requested by January) I’ve started thinking about what our membranes can offer extracorporeal membrane oxygenation, or ECMO. This post will begin with some background information that I think is particularly important and progress into what work I’ve done so far on…
Staph Aureus Immobilized in Microchannels
I have been developing a microsystem for Paul Dunman’s group that will allow them to flow in bacteria, immobilize them on antibodies, and then subject them to varying concentrations of antibiotics. The full system will incorporate a fluidic mixer and a NPN membrane as a way to introduce the antibiotic I have been working with…