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Pore Processing Comparison of W615
In order to support the idea that RTP treatment does not change membrane pore characteristics (as shown by Anant in the rhodamine diffusion study) I processed TEM samples that were not treated, RTPed in Ar, for 5 minutes at 800C, and RTPed in O2 at 800C for 5 minutes (I believe this was not a…
5nm gold separations
I set up separations of 5nm Au nanoparticles in both H2O and KCl in a new system. The stir cell isn’t always reliable for separations due to leaking and contaminating (?) particles from the plastic. To perform these separations, I added 20ul of sample to a sepcon and wetted the backside of the sepcon with…
BSA detection on NC
Creating an array to hold a pnc-Si wafer to proving to be difficult. The PDMS spreads too rapidly though the NC to form a consistent array for the pnc-Si membrane (and eventually wafer) to rest on. I’m still running different trials of the PDMS on NC to see if I can get the array to…
Makerbot2 printed Transwells for SEPCON chips
In Nottingham this summer, I attempted to use a Makerbot2 printer to create culture devices for SEPCON chips. I had varying degrees of success, but I was not able to get a good seal between the PLA and gaskets using only machined grooves. This would have limited our ability to do TEER and other 2-chamber…
A New Update in the Particle Capture Saga
ByKileanIn this edition of the particle capture saga (this will eventually be an epic like Gilgamesh or Beowulf, we will start telling this tale by the fire and eventually thousands of years from now scholars will look back on this work and deem it a huge impact in the history of human evolution), I have…
Testing of Printed Transwells for SEPCON chips
Testing was performed on 3D-printed transwell device prototypes for use as culture devices with SEPCON chip integration(Makerbot2 printed Transwells for SEPCON chips). The performed testing attempted to determine the quality of seal of interfaces between all device components. The two devices tested were identical in basket geometry, with the only differences being in printing material….