Journal Club: Preview from Sabrina Leslie – CLiC with flow

Working on the development of PEG microarrays for cell heterogeneity applications. Current work has been accomplished on TPM coated glass coverslips. Transfer to membranes are in progress. Three different x-linker concentrations were tested in a 1:1 mixture of PEG and DI or PBS. We are testing for what will make the most optimal cell culture…

Hi all, the technique of UV Ozone bonding (of PDMS to glass, Si or pnc-Si) is now complete, serendipitously. Hsin from Miller lab want to bond PDMS to glass, they tried plasma oxidation treatment and it worked.  The method is as followed: plasma oxidation treatment for 35 sec (which did not bond the sample, when…

Hopefully in the next week we will be submitting the following manuscript … X. Li, D. Johnson, W. Ma, H. Chung, J. Getpreecharsawas, J.L. McGrath, A.A. Shestopalov, Modification of Nanoporous Silicon Nitride with Stable and Functional Organic Monolayers, ACS Chemistry of Materials  (2016) submitted. Here are the highlights … Traditional silanization of silicon materials uses…

In order from left to right: 1) t=0, huvec on pncSi, in EGM (10% FBS) 2) t=24 hrs (cells reach confluence) 3) DIC after staining 4) FITC (live cells) 5) Rhodamine (dead cells) Its good to see the cells reach confluence and the membranes intact at the same time! There is a decrease in number…

The urea assay is straight forward. 6 wells are filled with 0, 1, 2, 3, 4, and 5 nmol of the urea standard (50 nL). Each well is activated with 50 nL of the reaction mix. Reaction Mix Sample control Urea Assay Buffer 44µl 46µl OxiRed Probe 2µl 2µl Enzyme mix 2µl 2µl Developer 2µl…

We desire to test the electrical perfomance of our membranes in the iBidi chamber. For comparison, I am using a gold standard Ussing chamber format to examine the contribution of our membranes to the overall frequency response of a system. I am using both a matlab model and a web-simulator (www.circuitlab.com) to examine an equivalent circuit model…