Maximum stability in cell culture conditions

ByAnant Agrawal

This experiment was carried out to determine the maximum life of pnc-Si in cell culture conditions based on discoloration. All the discoloration slowing factors were put together to see how long would the membranes last for. These include: samples in 3D format (Sepcon) with ‘membrane side’ down (with no intact membranes), all in incubator, DMEM (+FBS), all autoclaved, RTP’d and Silanized.

For both ‘RTP+Silanized’ samples, Silanization is done after RTP.

The samples (from W683) were compared with appropriate controls and were taken in duplicates. The media was changed every 3-4 days. (note: no cells present)

Observations: The 2D non treated samples (-RTP,-Silane) were discolored completely by 3 days.

The 3D non treated samples started to discolor after 5 days.

After a week, images were taken and as it can be seen, non treated and silanized (-RTP,+Silane) samples in 3D are almost discolored.

After 10 days, non treated and silanized samples in 3D discolored completely. Whereas, RTP (+RTP,-Silane) and ‘RTP+silanized’ (+RTP,+Silane) samples in 3D were still intact. The ‘RTP+silanized’ samples in 2D did not start discoloring either.

After 2 weeks, images were taken and as seen, the ‘RTP’ and ‘RTP+silanized’ samples do not show any discoloration. (A good news!)

This study is still going on and Barrett will take over in my absence.

Following is the updated chart after 7 weeks:

picture-14

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6 Comments

  2. Please repeat this study while you write/defend. We need to see if there are procedural differences between yourself and Barrett. The difference might be old material vs. AJA.

  4. I reckon, the wafer w683 that I used here doesn’t have any visible pores! This could also be a difference between my results and Barrett’s for ‘maximum stability’ check. Also, it seemed w683 was more amorphous in character and had too many pinholes.

  5. Barrett also used the ‘high’ bicarbonate media (3.7mg/ml). Hence, bicarbonate is not the difference. He also used samples with broken slits for his experiment.

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