Committee Meeting presentation

Tom came up with this idea of using these membrane with precise thickness and pore size control to obtain a critical thickness and pore size, beyond which neutrophils cannot go transmigrate. N-Formylmethionyl-leucyl-phenylalanine (fMLP) was used in the lower channel to serve as a chemotactic factor for transmigration. Additionally, for membranes with pore sizes or thicknesses…

With the help of Henry and Greg, the Fisher team has been busy learning the basics.  John and Ryan have learned some PDMS microfluidics-making and will have their first chips ready on Tuesday.  We have also been playing with the silhouette printer, studying our options for producing some simple devices.  Finally, we produced some chip designs to…

I reported last week that a 15 nm silicon film deposited with 05 W substrate bias yielded a highly porous film. This week, I wanted to confirm this effect as well as compare it to films deposited at 10 W and 25 W (our baseline). We find that the 05 W is reproducible and the…

We have long maintained that we can capture more analyte from a sample using a flow-through configuration compared to a tangential configuration. We can confirm this view quantitatively by measuring the amount of material captured on the membrane with equal source flow rates. Additionally, one of the advantages of a silicon-based platform is that we can…

Introduction In response to recent requests by our reviewers, I am looking to address two main concerns: 1) How does a collagen gel backing affect leukocyte transmigration, and 2) How does shear influence leukocyte transmigration. While we believe these areas are rich in information and need a significant amount of focus to tackle, we do…