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Silanization and BSA coating
Surface treatments like silanization were seen to slow down discoloration in the past. I carried out liquid amino-silanization (protocol in place) of samples from W683 (no RTP) and used them in both 2D and 3D format to monitor discoloration in DMEM(-FBS) @ 37C in Oven. Also BSA coated samples were used side by side for…
VEGF expression on pnc-Si transwell
All of my recent posts on VEGF, VEGFR2 and NF-H imunofluorescence have included data from cells grown on coverslips only. This was my first attempt to try to do immunofluorescence staining on intact pnc-Si transwells and then pop the chips out of the transwell plastic in order to image at 100X. Since VEGF is involved…
Residue on SiO2 membranes is dependent on wafer lot, but not storage location
Last meeting, we discussed the possibility of the storage gel-boxes outgassing a residue onto the porous SiO2 membranes, as evidenced by cells not adhering to the membranes and difficulty in acquiring SEM images. Possible Gel-Box Outgassing on Porous SiO2 Membranes Several cleaning methods were utilized in attempt to clean the membranes, such as O2 plasma,…
Hindrance is magnified in thicker membrane
Using the same procedure as in the last post, I was able to simulate a 6um thick membrane with the same pore characteristics as the experimental pnc-Si membranes. Material diffuses more slowly through this membrane, but not as slowly as I expected. The following figure shoes the filtrate/retentate concentration ratios at 24 hours for a…
Using Alumina Deposition to Modulate Pore Size
The Cambridge Microsystems Savannah 200 atomic layer deposition (ALD) system uses a two-step chemical process that alternates pulses of water vapor and trimethylaluminum (TMA) to build a layer of Al2O3 on a silicon substate. Because of the two-step nature of the process, the film is forced to grow by successive monolayers, each with a thickness…
Spreading of HUVECs on Dimpled Sylgard-184 Substrates
To analyze the dimpled Sylgard substrates that we’ve been making, we’ve been conducting experiments to investigate how topographical differences in the surface of a substrate impacts HUVECs and ADSCs, in this case the dimples. We’ve already shown that cells will grow on these membranes in our “Dimpled Sylgard Membrane Device” post and that our substrates are wetting in our “Degassing Experiments”…