Similar Posts
Long Term Urea Clearance in Serum
I made 50 mL of 30% fetal bovine serum (FBS), 70% PBS, 0.5 mM urea. Pumped with syringe pump at 5.6 µL/min through an untreated and EA treated single slot dialysis chips in a stirred beaker of PBS (two separate experiments). We expect (without Serum) a 30% extraction so from 0.5 mM urea to 0.35…
Desalting: Intact SepCons
Introduction One area of use to explore for the membranes is desalting, the removal of salts from solutions (such as those containing proteins). Ideal membranes have uniform pore distributions, high mechanical strength, tunable, sharp pore cutoffs, and high porosity. In this study intact samples from W565, which showed no air permeability but allowed rhodamine to…
Discoloration – Water soak
This week, I did a quick discoloration experiment based on Chris’s comment at lab meeting. He wondered if soaking the chips in water for a while would allow potential discoloration-causing contaminants to slowly leech out. I took 2 chips from wafer 622 and soaked them in ~ 300mL DIH20 overnight (with a couple of water…
Endothelial Focal Adhesion BMES Abstract 2015
The following post is the abstract that I recently submitted for the BMES Annual Conference. Introduction: Permeable membranes provide a physiologically relevant cell culture substrate for in vitro study of barrier cell types, such as endothelial cells, which assume a polarized morphology with distinct apical and basolateral surfaces. Cell morphology and subsequent monolayer formation and…
Update on entropy tricks with NPN
Since my last update things have been going quite smoothly. Most pores I make are able to pass 3 or 4 samples before clogging, and I have filled in the various graphs quite nicely. I still would like to flesh out the low and high end of the DNA length spectrum, but I’ve started writing…
Using Alumina Deposition to Modulate Pore Size
The Cambridge Microsystems Savannah 200 atomic layer deposition (ALD) system uses a two-step chemical process that alternates pulses of water vapor and trimethylaluminum (TMA) to build a layer of Al2O3 on a silicon substate. Because of the two-step nature of the process, the film is forced to grow by successive monolayers, each with a thickness…