PhD Committee Meeting and Internship with Sartorius
Hi all,
I have attached my presentation for 6 months PhD committee meeting explaining my internship project with Sartorius.
Best
Hi all,
I have attached my presentation for 6 months PhD committee meeting explaining my internship project with Sartorius.
Best
Jirachi has been working to make thin nafion films using our membranes as a support structure. Nafion is polymer that contains a sulfonate group, and as a result acts as a proton wire, allowing for cations (+) to be transported across a membrane, but not anions or electrons. Such a membrane can be used for filtering a…
New Lift-off setup, thanks to suggestions and concerns about flatness of the membrane after lift-off. Floating SU8 – SiN membrane with superstructure submerged. Membrane after removal from water Membrane, dry on first version of lift-off super structure. Membrane after separation. SiN has adhered to the superstructure and the SU8 peeled off the top. The…
The other (see below) just-to-be-sure experiment I performed this week was to compare staining between 2 different live cell dyes. I typically use calcein AM to stain live cells green. This is a membrane-permeant dye which, once inside cells, is hydrolyzed by intracellular esterases to make it fluoresce green. Another very common live cell stain…
For the charge model, we would like to know the surface charge density and size of our experimental molecules. I’ve attempted to use the Zetasizer for both measurements, but as mentioned in my diffusion paper, DLS gives sizes that deviate from the expected or crystal sizes. Since zeta potential measurements require large amounts of highly…
I retested the 100K Microcon, 100K Nanosep, and 300K Nanosep membranes at 1000RPM, which in the clinical centrifuge is 4.33psi by my calculations. I retested the 100K Nanosep just to make sure that we had some solid permeability values. I retested the 300K Nanosep because of the extremely low permeability I calculated for the first…
Hello Everyone, Here is an update of our meeting in Strong Hospital: So They are working in an urology lab, We met Sam who is a technician in Dr. Carla Beckham’s lab, he is responsible for cell culture part of the project. The cell line that they are working with is T24 with 80 -95% confluency….