R61: Slide Decks for 6 Month Progress Report

One of the validations of the in vitro human Blood Brain Barrier system is to ensure that the endothelial cells derived from iPSC’s and cultured in our system exhibit behaviors that are consistent with endothelial cell behavior in situ.  The particular aspect of BBB endothelial function addressed in this post is the expression and properties…

IntroductionThe ability to continuously sample and assay for the levels of soluble factors without disrupting the microenvironment is important to acquire sequential secretion profiles in BBB experiments on an uSIM module. Combining small volume sampling, buffer replenishment and sensitive pedELISA technology would enable longitudinal assays of proteins introduced to blood compartment or released from cells….

Introduction Breakdown of the blood-brain barrier (BBB) is one of the earliest signs of sepsis and is linked with long-term cognitive impairment. As animal models poorly recapitulate human sepsis, alternative approaches are direly needed to understand sepsis-associated brain injury and develop strategies that protect against cognitive decline. To fill this gap, we are developing a…