R61: Slide Decks for 6 Month Progress Report
PDF with all slides: R61 HL154249-01(6 Month Progress)
PDF with all slides: R61 HL154249-01(6 Month Progress)
This is the third installment in a series of posts focused on the development of a method for measuring barrier permeability in the µSiM. In Part 1 we discussed the goal of making these measurements in situ at a position beneath the membrane. In Part 2 we validated this approach experimentally on the Andor Dragonfly…
Introduction The capability of the m-μSiM for establishing an hiPSC-derived blood-brain barrier (BBB) in bioengineering and bioscience laboratories has been shown previously [1]. To further demonstrate interlaboratory agreement and successful dissemination of components and protocols, m-μSiM was used at RIT (Abhyankar lab) to establish an hiPSC-BMECs monolayer. Method All reagents and steps were used based…
Introduction Sepsis refers to an uncontrolled systemic inflammatory response which can result from bacterial blood infections. We have long been interested in developing a sepsis model using the μSiM-BBB platform because the neuroinflammation stemming from sepsis can cause severe BBB disruption. For the majority of our stimulation experiments we have relied on cytomix, a cocktail…
Introduction: Many applications of the µSiM require fluid to be continuously supplied to the microfluidic devices. We use laboratory tubing (typically Cole-Parmer/Tygon Tubing with I.D = 1/16″) that is connected to syringes or peristaltic pumps to do so. However, interfacing the tubing with the current entry and exit ports for the bottom channel is not…
AlineFlowBottomChannelSup AlineFlowBottomChannel
Slide deck from Large Group Meeting 12-21-12 (PPT) Access requirements for the AFM cantilver to contact the cells requires the cells to be grown on the membrane surface in what is typically the bottom channel of A-line devices (Typical A-line configuration is shown in slide 2). A PDMS block is attached to the well side…