Monocyte Isolation via CD14+ Selection (Protocol)
Please see the attached .PDF file for a full monocyte isolation protocol. This is for isolating human monocytes from whole blood.
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Wetting of Nanocavities using Isobaric Cooling
Summary and Instructional video The simplest way to get something wet is to allow water to condense on the surface of the something. In our case, we have a number of nanocavities (~ 0.1 femtoliters) with porous tents over them. The easiest thing to do is simply chill the substrates down to allow condensation to form….
Multiplexed sequential brain endothelial cell cytokine secretion measurement from μSIM
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in situ Permeability Analytical Solution Summary – Unhindered vs Hindered D
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A Power-free Pneumatic System for Controlling Fluid Flow
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Collagen IV is deposited below human umbilical vein endothelial cells
Introduction My previous post focused on attempts to reproducibly generate collagen IV with human umbilical vein endothelial cells (HUVECs) so that we would be able to establish protocols for basement membrane protein detection and degradation measurement. Following my presentation on November 18 2020, there were some questions regarding whether or not the observed collagen IV…