This is preliminary data for air permeability in track-etched membranes for membranes with different pore radius.
For comparison here is a table of the performance characteristics for TE membranes
Measured data is of the same order as official TE membranes data.
These Adarza chips were pre wetted with PBS then incubated with fetal bovine serum (FBS) overnight in the refrigerator then rinsed. TRITC (2 mg/mL) added to chips and incubated at room temperature for one hour. Then rinsed once with reaction buffer then rinsed thoroughly with distilled de-ionized (DDI) water. I first measured using the…
There’s been a flurry of activity regarding Jess’ recent discoloration post. Specifically, there is a discussion about the recent materials changes that have taken place due to the rebuilt CVC sputterer. This post is meant to supplement the ongoing materials discussion. I have noticed that films deposited post-rebuild seem to have less background texture and…
Since HUVEC morphology hasn’t looked good in short-term adhesion assays recently, I analyzed HUVEC on the 2nd day after cell seeding. Normally, CMFDA staining is done for 30-45 minutes in serum-free media. I wondered if the serum-free environment during staining was affecting the HUVEC. So, I stained one sample with 5uM CMFDA in serum free…
I’m currently trying to compare separations between different complex protein mixture. This was my first crack at depleted human serum. This mixture has been processed so that the most common blood proteins (such as albumin and immunoglobulins) have been removed. All three trials were performed using membranes from w338 in diffusion mode. 338 has a…
Introduction We are aiming to make device assembly easy and quick, particularly for distribution to non-engineering laboratories. We are collaborating with ALine to develop assembly via pressure-sensitive adhesion techniques. Assembly Process Modular Device Assembly Animation2 We made ten devices with porous membranes and ten with non-porous membranes to show no leakage. Current Issues and Potential…
In an effort to conserve membrane samples I am working to run diffusion experiments with a mixture of BSA and DDM. This will only use 2 chips instead of 4. In our meeting on Tuesday it was mentioned that BSA can be detected using the TECAN at 280nm, while DDM is not detected at that…
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At what pressure was the data in the table acquired?