PES protein separations
This was the first try at using PES for protein separations by diffusion. 100kD cutoff Nanoseps were used to separate 10mM KCl and 100mM KCl standards. In both cases, the backside fluid was gone after 24hours. I don’t think evaporation was the problem; either the membrane sucked up the fluid or it was drawn up to the retentate. For collection I pipetted 20uL on the backside and left it there for 10min in case I could pick up any residual protein.
The gel shows that there was nothing in the filtrates. However what is most noticable is the reduction of the species across the board in the retentate. I would hazard to guess that we’re seeing the loss right here. The next two charts hopefully illustrate the loss. I added the retentate to the filtrate (in this case the filtrate was just some noise) and compared that to the starting sample.
These differences are much bigger than those shown for pnc-Si membranes.
This looks great Jess. Remind me did we do this with cellulose membranes yet?
Cellulose membranes are running right now. I should have some results later today or tomorrow.