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ZO-1 Staining on ARPE-19 Cells grown on MgF2 nanomembranes
The following images are from ARPE-19 Cells cultured for 3 Weeks over here in Nottingham (thanks for help from Emilia Moradi, Victoria Ciampani, and Kevin Webb). MgF2 nanomembrane chips were positioned into a transwell insert and sealed using PDMS, a biocompatible polymer, creating a two-compartment transwell. After an ethanol sterilization, ARPE-19 cells were seeded in a 12 well…
Lift-Off Grant Update 01.12.16
160112 LO Grant Update
Histograms of recent membranes
Using Mike B.’s MATLAB script, I was able to generate some histograms and stats for wafer 187, 310, 319 and 320. I excluded the “small pore background”, as I do not believe these features go all the way through the membrane. wafer 187 wafer 310 wafer 319 wafer 320 The reason why I did not…
(Almost) Final Versions of Model Paper Figures
We’re submitting the following paper to Separations and Purifications within the next two weeks: model-paper model supplement Below are the (almost) final versions of the figures from the paper. Figure 1: Schematic of Particle Distribution for Particles Rejected by Membranes The distribution is a quasi-equilibrium between convection towards the membrane and back diffusion, which is…
Developing a Microfluidic Human Tendon-on-a-Chip to Investigate the Role of the Vasculature in Tendon Injury – MPS World Summit 2024 Poster
Isabelle Linares_MPS World Summit 2024_Poster
RTP Time Variation for Cell Culture
In an attempt to improve membrane life in cell culture, different RTP times were tried on a single wafer. Samples from W514 were RTPed in Ar at 800C for 1, 5, and 10 minutes, respectively. Cells were then seeded at confluence on membranes, with membrane stability monitored for breakage. Discoloration was monitored on samples without…