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Ozone and Contact Angles
The Ozone generator works very nicely. Dave is going to be providing an in-depth user manual for us, so I’ll be somewhat brief. In this first picture, I compare an ozone treated sample against a control. Both ‘droplets’ are 3uL of DI water. The treatment protocol:– 3 min of O2 at to fill main chamber,…
SU-8 3000 Film Stress Reduction Attempts
Summary: Annealing to above glass transition T (200 C) doesn’t reduce film stress. Adjusting post-exposure bake protocol seems to reduce it. A short post to document my efforts at reducing tensile stress in SU-8-3000 coatings by annealing in vacuum to its glass transition temperature (200 °C according to MicroChem). This is in relation to the…
Contact area dictates early cell-substrate interaction for endothelial cells on porous membranes
ByAnaAbstract Endothelial cell attachment to porous substrates via focal adhesions is initially affected by pore spacing and not absolute porosity. Maturation of endothelial monolayers via cell-cell interactions occurred over the same time course regardless of substrate porosity, pore size and contact area. Cell-matrix interactions at maturity were dictated by substrate stiffness. Data [missing figure] Fig. 4 Endothelial…
Transmission Spectra of pnc-Si
UPDATE:Chris took the membrane sample and normalized with by the no spanning membrane sample. This was plotted against the theoretical spectrum of single crystal silicon. The curves have a similar shape, but the single crystal silicon has lower transmission. This may be due to the fact that it’s a perfect material or that there’s error…
Spot the Difference: Testing the Spatial Resolution of the Andor Dragonfly Confocal Microscope Using GFP Tagged Staphylococcus aureus and Nonporous Membranes
While imaging GFP tagged Staphylococcus aureus on our membranes within µSiM-CA devices, the question of whether or not we can differentiate between bacteria on the top and bottom of the membrane versus within the membrane has persisted. We know that during our experiments bacteria exist in all three of these places, therefore the question really is whether…
Water Permeability Figure
Here is the figure we talked about in the last meeting. For each specific membrane, I’ve plotted both the experimental permeability and the permeability calculated from the theory. You can see in some cases they are close, but in others they can be pretty far off. Omitted from this chart are retests. Updated: Graph is…