Developing a Microfluidic Human Tendon-on-a-Chip to Investigate the Role of the Vasculature in Tendon Injury – MPS World Summit 2024 Poster

Hi everyone, One of the objectives in the last aim of my PhD is to investigate the effect of soluble factors secreted by adipose derived stem cells (ADSCs) on wound recovery of fibroblasts (FBs). We have categorized these soluble factors to 3 subpopulations including: large-EVs, small-EVs, and non-EV associated molecules. For the last 3 weeks,…

I’ve tried to get some more data for the phenomenum that may be electro-osmosis in our electrophoretic cell.  In the last post I looked at the rate of passage of 1x PBS in pinhole membranes from w621.  After that I first tried to see if DI H20 would also work (also pin.  At 15V (which…

Molar flow thought pores with different diameters under 1 psi. First, I calculated molar flow based on porosity histograms extracted from TEM images. And then compared it with the actual experimental data. Here are few examples of this. molar%20flow.ppt

My previous post outlined the results of a EQ format separations using membranes from wafer 319. I have followed up that experiment using the same membranes, but in a diffusion based separation. I separated 2uL of 9.3 mg/mL clarified brain extract using a reservoir of 60uL. I allowed the diffusion process to occur overnight, and…

A few of the newest SC wafers came back with intact samples without any pinholes. First here is a list of the experiments that we want to do in our lab with intact/pinhole free membranes 1. Protein separations – Requires >10 samples per wafer (preferably even more) for replicates of separations in both pressure and…

This post presents cell images from the first successful co-culture experiment.  I seeded P8 bEnd3 cells alone, P13 NG108-15 cells alone and both cells on PET and SC500 pnc-Si transwells.  For this experiment, I seeded 50,000 bEnd3/cm2 on the apical side and 50000 NG108-15/cm2 on the basolateral side of the transwell.  I tracked TEER (future…