Manufacturer’s Data
What is SiR-DNA? It is a far-red DNA stain, known as SiR-Hoechst, and can be used for live-cell imaging. Most current DNA dyes are toxic or affect the cell’s motion or proliferation. https://www.nature.com/articles/ncomms9497 It does not affect the migration of human dermal fibroblast cells (hdFb). The motility parameters (speed and displacement) for a 24 hour…
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Last week, I reported on the the morphology of pnc-Si deposited with different substrate biases. We hypothesized that the reason for this was due to the different initial film stress of the as-deposited a-Si. Unfortunately, the pressure-displacement method does not allow us to measure amorphous films since it requires the membrane to be nearly flat…
Traditional Wound Healing Analysis (the implicit gold standard) Wound recovery is reported base on the closing of the two wound edges. Proposed Wound Healing Analysis (cell counting/tracking methods): Wound recovery is reported base on the migration/movement of the wound healing cells. Recap on angle autocorrelation We look at the correlation of angles between…
Last week we processed wafers 335 – 340. Our goal was to track down the source of Cu contamination. We sputtered membrane films both at room temperature and at 400 C. Our thought was that the RT dep. would not show contamination if the sputter heater was indeed the source of our Cu. It turns…
I went over to RIT to work with JP on measuring the deflection of NPN and pnc-Si membranes as a function of pressure. JP and Dave Fang did this work years ago, and we want to pick it up again to correlate with Sarah’s burst pressure data. We used the Veeco Wyko (white light interferometer)…