The future of the Goergen Hall Basement
Coming to a basement near you by the end of 2008 (assuming no delays that is). Pretty exciting in my opinion.
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A Move in the Light Direction: Visualizing Staphylococcus aureus Division Across Membranes Within µSiM-CA Devices Using Confocal Microscopy
Recently, several studies on Staphylococcus aureus (S. aureus) have been hindered due to poor performance from assays using the µSiM-Canalicular Array (CA). As discussed here, we attempted to improve assay performance by coating our membranes with fibronectin. This did not work well and instead left us in a state of confusion as we did not…
Designing a Reverse-Centrifugation Sepcon Variant (Part 1: Geometry)
We have an STTR grant funding us to revise the Sepcon plastic housing to enable us to do reverse centrifugation easier. As outlined in this previous post, we’re trying to remove unbound IgG from a solution of IgG-antibody conjugates using reverse centrifugation: The earlier work used Josh Winan’s jury-rigged PDMS-filled reverse centrifugation setup, but despite the…
Particle Tracking in Tangential Flow Analysis
ByKileanWhen we are trying to optimize the capture of exosomes in the tangential flow system, it is important to understand how particles are behaving in the flow. Initially, we tried to do this last summer by looking into the particle tracing package for COMSOL, but that required a complete overhaul of the software (a new…
Less is More: Lower Initial Bacteria OD Increases Transmission in uSiM-CA Bacteria Assays
On our quest to optimize bacteria assays using µSiM-CAs, we recently discovered that higher initial bacterial ODs decrease transmission in positive controls, described here. This was something we did not initially anticipate, engendering a new hypothesis around the idea that less initial bacteria leads to more transmission across our membranes in the µSiM-CAs due to…
The effect of Exosomes on Wound Healing
ByAslanHello Everyone, The goal of these experiments was to study the effect of exosomes on wound healing. We wanted to study, first importance of exosomes for migration of FBs and eventually closure (healing) of the wound and second effect of exosomes derived from ADSCs on FBs migration. In order to test our hypothesis, we designed…
Concurrent TEER and Phase Microscopy with Yuchen Wang
For the first time, we did time-lapse phase microscopy (1 frame / 5 seconds) looking at endothelial cells through the ITO in the static BBB device (device A bottom left well) and measuring the TEER concurrently. Here’s the video, just shy of 15 minutes of recording. (reporting a low resistance: 5-10 ohms, shorted). TEER Test…