UV-Ozone treated SC267: DDM passes and BSA held back

Building on my recent posts I ran an experiment with SC267 testing the ability of BSA and DDM to diffuse through UV-Ozone treated (20min at 150C) and untreated samples. I used four samples testing both BSA and DDM in the treated and untreated cases using Jess’ diffusion cell. Again 6uL was placed on the membranes topside with 60uL of DI-water on the backside. Unfortunately when I checked the system this morning it appeared that the diffusion cell had been “bumped” becasue the untreated-BSA sample had been knock off of the diffusion cell.

DDM diffusion:

In both the treated and untreated sample the DDM was shown to diffused through the membrane. The final concentrations of the filtrate (diffusate/permeate) were calculated to be approximately 0.15% and 0.14%. In the assay image the bottom row first well is the untreated SC267 sample and the second well is the treated SC267 sample.

BSA Diffusion:

Since the untreated BSA sample was compromised we are going to be rerunning this part of the experiment to verify that we can see BSA diffusion across untreated samples. On this note we are out of SC267 samples so I would propose further testing on SC269 samples. In the treated BSA sample the concentration of BSA in the filtrate (diffusate/permeate) was calculated to be essentially 0mg/mL. Again in the assay image the bottom row the first well is the untreated sample (compromised) and the second well is the treated sample.

From this experiment it appears that treated SC267 samples allow for DDM to diffuse across the membrane while still preventing BSA diffusion. In the case of the untreated samples we know that DDM can diffuse across the membrane but we need to verify what I saw last week; that BSA does diffuse across untreated samples. Justin will be running this section of the experiment within the next few days.