Vapor based PEG deposition

Originally, I created the following figure to visualize the relationship between sieving coefficient and molecular weight: (originally from this post) I can now take the protein dimensions that I obtained from crystal structures (posted here) and replot this figure: In this version of the figure the sigmoidal shape of the curve is even more obvious…

  Overview I burst pressure tested TEM chips with 1×1 mm windows of 100 nm SiO2 that were both annealed and unannealed (provided by JP and Josh Miller). In our experience it seems that the forming gas (H2/N2) annealed chips are stronger and never wrinkle. These burst pressure tests were performed in an attempt to…

My previous 2 posts on co-cultures presented images but I also tracked the TEER in these experiments.  This post shows that data. The top 2 graphs are from the 1st co-culture experiment – the bottom graph is from the second experiment (where my PET controls died), as % Day 0: On PET, the coculture TEER…

I set up diffusion experiments using the wafers that Tom was able to pass gold through in DI H2O using pressure.  5nm particles do not pass in 24 or 48 hours.  If we have extra samples I will be testing 5nm particles in 10mM KCl.  

I’ve tried to get some more data for the phenomenum that may be electro-osmosis in our electrophoretic cell.  In the last post I looked at the rate of passage of 1x PBS in pinhole membranes from w621.  After that I first tried to see if DI H20 would also work (also pin.  At 15V (which…

There has been some confusion lately with what we should name the compartments in diffusion.  Here’s what I’ve come up with: Top/Compartment with initial sample: Retentate Bottom/Compartment to which things diffuse: Dialysate The original word we were using for dialysate, filtrate, implies an active process and was criticized by scientists at NAMS.  While dialysate implies…