Endothelial Vacuoles Induced by Flow
In working on a long over due paper by Barrett on endothelial response to high permeability material …
Key figs …
And one post from Nakul.
I came across this highly relevant paper
Last week, we created a series of wafers that were RTPed at four different temperatures between 900 C and 1000 C. Below is a comparison of the 900 C (left) vs. 1000 C RTP (right) wafer. We see that a difference of 100 C shifts the cutoff ~10 nm. The pores are also the roundest…
This is just an update for the experiments Tejas and I have been working on for the past two weeks: First, we tried incorporating 20 μl of Geltrex (10 mg/ml, diluted with DMEM from stock concentration: i.e. 15.6 mg/ml) in the back of a non-porous chip with 0.02 μm blue fluorescent nano-spheres (1:10 dilution from stock concentration)….
Barrett had discovered that growing endothelial cells on pnc-si or tranwells causes vacuoles to form. The vacuoles on pnc-si formed only on the free standing areas of the membrane where there are open pores. The background on pnc-si chips showed negligible vacuole formation. The density of vacuoles on pnc-si was almost two fold higher than…
I have wondered if it were possible to create free-standing, nanoporous nanomembranes out of other materials (rather than my MgF2 process). The process is general enough that it should work. The major factors are Etch Selectivity between the substrate and the deposited material Film Mechanics (some films tear themselves apart due to the relative sizes…
http://www.genengnews.com/insight-and-intelligence/silicon-ideal-to-culture-patient-on-a-chip/77900627/ Organs-on-Chips Remotely Monitored, Controlled via Google Glass
Last Friday, Chris and I met with Dave Glocker from Isoflux Inc., an expert in physcial vapor deposition. Specifically, we wanted to get advice on the recent problems we’ve had with the AJA tool. Arcing from Lesker targets We recently decided to try new oxide targets from Lesker because the last batch of AJA targets…