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Confocal movies of BBB co-cultures
Over the last couple of weeks, I’ve been doing confocal fluorescence microscopy over at the Med Ctr. I was hoping this tool would clean up some of the background fluorescence in my immunofluorescent images. More importantly, the confocal can take ‘slices’ of images in the z-plane and stack them on top of each other to…
Streaming potential -> Zeta potential
In this post I will describe recent streaming potential measurements for PES membranes and the calculated apparent zeta potentials from these measurements. Setup: 1. Fabricate Ag/AgCl electrodes: Silver wire at the anode in 1M KCl at 1mA current for 20 minutes. 2. Assemble streaming potential device: Electrodes are inserted into silicone chord o-rings and chamber…
Cytochrome C polymerization
ByJimWe have often encountered cytochome C dimers as part of our work. For example in this figure from Jess that we often refer to in our hemodialysis work. It turns out that Cyto C polymerization is a well known phenomenon. The dimers are very tough and survive the denaturating conditions in Jess’ gel. Here is…
Notes from the 2015 ECM4 Conference, Aberdeen, Scotlandd
Notes from the ECM4 conference Met with Mathew Halls from Schrodinger. He was curious about what I did, came up, gave me his card. Apparently he knows Chin Tang. He’s giving a talk on the last day. Opening Remarks – Bob Collier – Aberdeen Chamber of Commerce European Capital of Oil and Gas. 25% of…
Wafer #250409-13 – 0.5 μm Microslit
Manufacturer’s Data Logged by: Ahmet Gurcan Membrane Type: MSSN 0.5 micron Microslit SKU: MSSN400.C-3LX.1-0.5E1 Lot Number: 250409-13 Quantity Received: 250 Manufacturer Test Report: Flow Data Avg in SLPM 4psi: 3.96 15psi: 14.4 Burst: 9.82 PSI
Initial Nanofluidic Transistor Zetapotentials Seem Unresponsive to Applied Gate Voltage
I’ve been taking lots of zetapotential measurements with my current setup. Serendipitously, it appears that the more I use the electrodes, the more stable my zetapotential measurements become. Because the system seems well behaved now, I decided to move on to a more difficult problem. Sarah and I made some nanofluidic transistors a few weeks back,…