Aline Devices for Cell Culture

fornasiero2008

In this post I’m trying to give a better comparison among filtrations using chips from wafer 1085 using 20 and 100 nm fluorospheres, IgG, and 5 and 20 nm Au NPs.  In each slide I try to indicate the flow direction with respect to the membrane.  Unless I’ve written “Pressure” near the direction the separations…

Tom prepared two PVP coated TEM samples for me to image today. One of the samples was washed with dH20 and the other left untouched. Below is a series of three images, from left to right: no PVP, PVP no wash, PVP & wash. I wasn’t able to tell any differences under the River Campus…

Demos of the Molecular Devices M2e and Tecan Infinite M200 are done. Erik from Tecan left us the M200 to use until October 6th.  Please come try out your assays on it.  Or check sensitivities.  Jess or I could probably steer you in the right direction as far as the software is concerned. My impressions…

As Jim suggested, we are not only going to reproduce what Chris and Tom did in the Nature paper but also trying to test the charge screening effect based on different salt concentrations. To do such experiments, we need to charge our Si membrane and at the same time oxidize it. At the end of…

Thanks to our collaborators at the University of Chicago we have now used NPN to demonstrate sodium/hydrogen exchange functions at the level of single EVs. This is first-of-a-kind evidence of protein function in individual EVs. It will be officially published in Communcations Biology and will soon be located at this DOI. What follows are key…