Gene vs Protein nomenclature

In an attempt to help wet the pores prior to permeability experiments, I’ve started soaking assembled SepCons in a %50 ethanol solution.  Each SepCon is inserted into an eppendorf so that the membrane side is fully submerged.  I realized that in my last post, I was soaking multiple samples in a beaker which did not…

  I have been looking into PLA (Poly Lactic Acid) plastic for use in our microfluidics. PLA is a commonly used material in 3-d printers, so it would be great if we could print out our microchannels for cell-culture devices. To that end, I wanted to see if the PLA plastic would react with our…

In our prior work we showed that endothelial cells form far fewer focal adhesions on microporous SiO2 membranes compared to non-porous SiO2 and tissue culture plastic (see prior post by Ana). We performed this analysis at 24 hours, which seems to be the standard in the literature. Interestingly, we found that the number of FAs…

I got three pinhole-free membranes from Karl after the inspection. They are from wafer#612. Locations are (-3, 1), (-3, 2), and (-3, 3). I used the UV/Ozone machine to oxidize and charge two of them while leaving the third one as a control. The protocol of UV/Ozone treatment was followed. The stir cell was set…