Author: Alec

Here are the figures for Tejas’ paper that is in the final stage of drafting:

Introduction In a previous post I introduced the concept of endothelium apicobasal polarity and the potential to utilize this concept for future sepsis drug development: Endothelium Apicobasal Polarity Regulates the Innate Immune Response In Vitro [In Progress] In this post, I will expand on the ‘mock’ TNF-α experiments with recombinant IL-8 as well as add…

Introduction Etaluma is a company that builds high resolution microscopes in a compact format for integration into cell culture incubators. This shifts from the traditional stage top or incubator cage approach to live cell imaging to allow for steady time-lapse imaging over extended periods of time without issues of focus or worries of temperature or…

Introduction Currently, no FDA approved drug exist for the treatment of sepsis. Past sepsis pharmaceuticals attempted to target mediators of the innate immune response. These drugs, however, struggled to balance remedial immune suppression from complete immune knockdown in a patient population plagued my a maladative immune system. This fundamental issues is due in part to…

Here are my proposal exam materials for reference. Proposal Document: Proposal_Salminen Proposal Presentation (No Animations or Videos): Proposal Presentation_No Animations

Introduction In response to recent requests by our reviewers, I am looking to address two main concerns: 1) How does a collagen gel backing affect leukocyte transmigration, and 2) How does shear influence leukocyte transmigration. While we believe these areas are rich in information and need a significant amount of focus to tackle, we do…

BMES – Atlanta 10/17/18-10/20/18 F-actin Alignment Quantification Using HSB Color Coded Maps ImageJ Plugin: OrientationJ Purpose: Quantification of F-actin alignment using ICC. HSB map provides a visual representation of fiber alignment with color codes related to hue:local orientation, saturation:coherency, brightness:fiber stain intensity (Figure 1). Our use: Quantification of HUVEC alignment before and after flow. Fluorescent…

Introduction In severe sepsis, a primary infection gives way to a maladaptive immune response leading to wide spread edema and multiple organ dysfunction. The initial infection can occur in many peripheral tissues throughout the body. The pathogen associated molecular patterns (e.g. LPS on gram-negative bacteria) interact with pattern recognizing receptors on resident macrophages and endothelial…