Updates: We can now put the glass on top of the mask, which allows the use of nonstick agents in the creation of PEG microarrays. The ability to cross-link PEG-Diacrylate (PEG-DA) with a photoinitator and UV exposure was previously tested and shown to work. At the lowest power setting on the Leica (~25%) fluorescence source, we were…
Yuchen and I have been tasked with integrating TEER electrodes into the Cytovu format. The general idea was to fabricate the TEER electrodes similarly to what I have done already, then add them to the current Cytovu device, using UV ozone bonding and PDMS (to cement in the top electrode). I drew some electrodes to…
On Friday Josh and I assembled Sepcon chips with calculated porosity of ~6.5 %, and avg. pore diameter of ~40 nm into Sepcons. I was able to pass 40nm gold through the pores (we don’t have any gold larger than that). Note the “Bad” retentate – for some reason one of the sepcons didn’t filter anything. On…
Please fill out this Lab Specific training form, sign and return to me. If the item is not applicable to your work, sign N/A. Jim LabSpecificTraining(2013).pdf
http://phys.org/news/2013-03-temp-controlled-nanopores-blood-analysis.html
EDIT: This problem was solved thanks to Tejas. It turns out that Falcon BD transparent 96 well plates absorb in the UV range, and are best used for bradford assays or anything that measures absorbance higher than the UV range. Apparently they sell UV-inactive 96 well plates, but they are very expensive. Josh Winans and…
The NRG website downtime you’ve suffered in recent weeks is paying dividends. Most of this has been in operation for a while, but in case you haven’t noticed here is the first round of changes we’ve rolled out … Sharing internal NRG News. If you have a note or news item to share with the NRG you…
Links and Papers: Article with video Lung on a Chip Device Paper Lung on a Chip Application (Pulmonary Edema) Chris came across the publicity video last week and Jim asked me to look into the specifics. It’s a pretty nifty device. As the video shows, the device is a co-culture structure fabricated entirely from PDMS….
We created a 25-40-25 OSO stack with a SEPCON pattern. RTP at 1050 C 100 C/s 60 s. The following images show a comparison between the STEM detector and the SEM detector, and then a couple more images at 100 kx and 50 kx. I calculated a porosity of ~6.5 %, and…
This week I tried looking into several different ways of detecting the elusive Barcikowski Peptide. We discovered that Coomassie staining does not work, both because of the size of the peptide (it only works on proteins with a MW > 3000Da) and because Coomassie preferentially binds basic residues (and with a sequence of CEEEEEEEEEEEEW, we’re…