If you know me at all, you know I enjoy nothing more than ultrafiltration and Parylene-C deposition. Now these activities are combined into one device to improve the biocompatibility (platelet activation and adhesion) of nanoporous membranes. In this letter, polyethersulfone (PES) membranes were coated with Parylene-C, poly(chloro-para-xylylene) aka (PCPX), while glycerin vapors rising through the…
We deposited a 20/60/20 nm OSO stack for an ellipsometry test. We decided to try annealing the un-patterned wafer in the RTP at 1050 C with no susceptor with a 100 C/s ramp for 60 s. The attached SEM images show pores forming. With these encouraging results, we are considering depositing 40, 50 and 60…
In collaboration with Alex Shestopalov’s lab, I started working on liquid based PEG treatment on windowed sepcon chips. The treatment is a 3-day long protocol and involves multiple washing and drying steps with different reagents. Most of our sepcon chips broke down in first few steps itself. The initial wash consists of Nanostrip (sulfuric plus nitric…
These Adarza chips were pre wetted with PBS then incubated with fetal bovine serum (FBS) overnight in the refrigerator then rinsed. TRITC (2 mg/mL) added to chips and incubated at room temperature for one hour. Then rinsed once with reaction buffer then rinsed thoroughly with distilled de-ionized (DDI) water. I first measured using the…
Josh and I are taking a closer look at how the samples burst and what different factors can affect the strength of the membrane. We are specifically looking at minimum dimension, area, and perimeter. A wafer was designed that contained different sized samples. One of the three factors were held constant while the other two…
In a previous post, we showed that both Cytochrome C and BSA at concentrations of ~1mg/mL through 20nm cutoff Sepcons with about the same filtrate concentration (~60% of the feed concentration). Yesterday (1/14/13) we repeated these experiments with 10 and 15nm cutoff Sepcons and demonstrated pretty clearly that either 10 or 15nm Sepcons can be used…
This is a summary of diffusion experiments with A3G that were conducted during the week of 5/10/2010-5/14/2010. The HMM structure of A3G is a large protein multimer held together with RNA. Since the ultimate goal of this project is to develop a screening assay for HIV drugs, a successful experiment is one that will show…
Hi Guys, I thought this might be interesting, especially with the interest in SiN filters. While playing around with the SiN membranes for my project over in ChemE, I fought that the 1 micron SiN is surprisingly strong and that I can place our silicone gaskets onto the membrane and remove it without damage. By placing the…
With the goniometer nearly finished, I’d like to share a look at what has been created: Introduction A surface contact angle goniometer (SCAG) measures the angle(s) of contact that a droplet of liquid makes with a surface. This particular SCAG does this by creating a shadow that is isometric with the droplet and recording its…
Before the fresh Cytochrome C arrived, I ran two samples. The first sample was our old Cytochrome C in 0.3mM sodium phosphate buffer at a concentration of ~1mg/mL. I passed it through the 20nm cutoff 3rd generation Sepcons by spinning it in the small centrifuge at 2000rpm for 5 minutes. Approximately 100uL of sample passed through…