A group published a paper in Lab on a Chip (2012) using track-etched membranes for electro-osmosis. There are a lot of eerily familiar terms and concepts in the paper. The title even sounds familiar: Low-voltage electroosmotic pumps fabricated from track-etched polymer membranes They created a device with metal mesh electrodes on the opposite sides of…
Josh has been kind enough to fabricate a shadow mask for me to create interdigitated electrodes without the hassle of photolithography and etching. In order to be able to use this method, I want to make sure the produced electrodes are the same or better than the old method. I worry about shadowing effects due…
pnc-Si chips prewetted with PBS then incubated with fetal bovine serum (unknown molarity) for 2 hours under refrigerated conditions. Thoroughly rinsed with reaction buffer (perfused 5 times). TRITC (2 mg/mL) added to dry chips and incubated at room temperature for one hour. Then rinsed once with reaction buffer then rinsed thoroughly with distilled de-ionized (DDI)…
Recall that we’ve been having a great deal of trouble following the free ligand during our separations of the Bioconjugated Barcikowski particles, and that this trouble also means we can’t see the ligand after centrifuging the pure sample. In the interest of thoroughness, I replicated the assay the Barcikowski lab used to quantify the unbound…
In order to verify the validity of the result of the five-layer structure I did last time, I deposited another wafer with NOSON stack, which has the identical thickness as that of the last time. The annealing condition is also same as the last time, which is 1 minute holding time at 1000C with a…
During my visit last, I deposited a couple of wafers and one of them is to test a even thicker Si film from the NSN stack. The thickness for the Si film is 200nm and the nitride layers are still 30nm. After deposition, the Si wafer was etched and the pop-out TEM grids were then…
The last of the larger windowless pnc-Si chips were sent to both Adarza and Dr. Shestopalov’s lab for functionalization. Adarza returned chips with PEG-1 and PEG-2 (different rounds of processing) with both 2 hours and 16 hour treatments. They also returned a set treated with an alcohol leaving group ,ethanolamine (EA). They did also supply…
One major problem that we faced with our current shear-free chemotaxis system is the lack of high image quality. Since the silicon-nitride (SiN )grid is 100 um away from the coverslip surface on which the cells migrate, the out-of-focused SiN grid blurs the image. This makes cell tracking much harder. An example of a movie…
To assess how shear-free our [ “shear-free” chemotaxis system] is, we tracked the motion of nanoparticles in the shear-free compartment as we input a known flow rate in the flow compartment. The nanoparticles we use are the 210 nm dragon green fluorescent beads. ==================================================================Following is a 6-min long movie of the nanoparticles flown at a flow…
Hi all, We have already showed in our earlier [ post ] that our shear-free chemotaxis system can indeed elicit chemotaxis, suggesting that we have successfully translated a gradient through the membrane to the shear-free side of the system where the cells reside. However, it is still nice to show via fluorescence how the translated gradient looks…