I have been trying to fabricate my own Indium Tin Oxide (ITO) electrodes on coverslips for the BBB device in order to minimize the working distance for any microscopy. Currently, I am using microscope slides that are way too thick (mm). Coverslips are generally about 0.2mm thick. I am using a combination of sputter, photolithography,…
Comsol modeling was done to find the ideal channel height and volumetric flow rate. What is clear from the simulation is that ever increasing flow rates in the model lead to increased clearance and decreasing channel heights give some benefit but not on the order previously expected. In addition, (Depner 2005) shows that increasing the flow…
A few weeks ago, I did some RTP annealing of NSN and OSO stacks. The purpose of this annealing experiment was hoping to capture the onset of pore formation and see whether there is any different behavior between NSN and OSO stacks. The annealing temperature was set to 700C. The ramp rate was 50C/s all…
The Barcikowski samples, sent from Germany a month ago, continue to be a fecund source of inconsistant data. Using the DLS to monitor several filtrations has resulted in a series of baffling results that I think I’ve finally begun to understand. First, the promising results: A DLS of Sample 1 (a mixture of bioconjugated gold…
I have created a successfully sealed flow chamber with this current device (V2.4), with the caveat that I sealed off the top chamber and was unable to test leaking from the top to the bottom. Again, the dialysis chips from Dean’s work were used, as well as the same bulk microscope slide electrodes. Improvements…
The CHA e-beam evaporator in RIT had been used traditionally for the deposition of metal on the pnc-Si membranes. The most important factor for metal deposition on the membranes is that the nanoholes must not be blocked. So the metal deposited needs to be smooth and continuous. Ag or Au does not adhere onto Si…
As we make larger and larger pores, we see that they are becoming more convoluted and have stopped looking like circles or ellipse. In order to try describing these shapes, they were given a “roundness” number which was calculated by taking the minor axis and dividing it by the major axis. However this often fails…
I’ve made some modifications to my V2 design such that it allows total vertical access, among other things: This design utilizes embedded electrodes and filter within gasket layers to create a tight fluidic seal for each channel. Here, the slot is pointed upward, so the endothelials will be on the bottom side and the…
In this experiment I use a syringe pump to move urea dissolved in ddH2O through a 10 mm long, 300 µm high, 500 µm wide dialysis channel. The retentate is collected in a fraction collector then measured using a urea assay from Abcam. Our first experiment on Urea clearance with BSA was performed with 0.5…
This study was run to test test the efficacy of PEGylation in blocking BSA adsorption. Three surface types were used, glass, PEGylated glass, and PEGylated pnc-SI. The glass was cleaned in Ethyl Alcohol, dried and UV treated, PEGylated glass was procured from Microsurfaces Inc. who also PEGylated our samples of pnc-Si, which did not have…