I took a sample from w304 over to the Krauss Lab and had them try to image it with AFM. Here are a few pictures (for some reason the color scales weren’t included in a couple of them, but they go from 0-dark to 5-light nm): In both images you can surprisingly see the pores….
I ran a series of serial dilutions with the rhodamine, to find the absorbency using the spectrometer. Below is a graph of the concentration of rhodamine versus the absorbency. I removed the concentrations which had absorbances greater than 1.7 AU because those absorbencies created a greater deviation. The lower concentrations show a more linear distribution….
Last week Jess helped me set up the diffusion cell with human albumin and cytochrome c. We ran 3 cells and used membranes from wafer 565. The membranes used were -1,4 (retentate 1), 3,3 (retentate 2), and 4,2 (retentate 3). The gel is posted below: The columns are (from left): Protein, control, retentate 1, filtrate…
As Jim suggested, we are not only going to reproduce what Chris and Tom did in the Nature paper but also trying to test the charge screening effect based on different salt concentrations. To do such experiments, we need to charge our Si membrane and at the same time oxidize it. At the end of…
Hi all, you might have read that we (Mike and i) have 100 % bonding efficiency in our latest PDMS-Si wafer bonding. On that same trial we have also successfully bonded PDMS to the new pnc-Si that Dave made for us (wafer 619, the rectilinear one with the V-shaped trenches leading to the Si-wafer well…
Hi All, Recently we have good results showing that we can bond PDMS to both glass and Si-wafer with UV Ozone treatment. We can even bond the PDMS to pnc-Si directly, although it is not clear whether the sealing is perfect. However, the success rate is not always 100% (either no bonding or partial bonding…
Last week I ran cell culture growth experiments on RTPed (800C 5 minutes in Ar) samples from W567 (single slit 100um x 1000um). For this study I tracked discoloration w/cells, membrane stability w/cells, and the bead assay w/cells to see if we can predict if a membrane used in cell culture is intact by monitoring…
Recently I tested track etched membranes and crystalline silicon (basically the backside of the membrane chips) following the sample buffer adsorption assay. This new data comprises the last two bars on the following chart. I’m surprised to see that crystalline silicon is so low in comparison to pnc-Si. This is something I’d like to test…
Time lapse movie of fibroblast spreading on the nanomembrane was captured over a period of ~ 8 hours. I was having some problem uploading the video here, thus have done so in the NRG knowledge page under the cell culture manuscript.
I’ve added a page on cell culture applications to the knowledge wiki. Watch this page and edit it if the topic is of interest to you. Remember the wikis provide a place to comment on the changes you make … and to compare and restore prior content … … so don’t be shy.