I ran another diffusion cell last week using Human Albumin and Cytochrome C. I increased the volume of PBS because the last time I ran the gel for this mixture, the band for cytochrome c was very prominent. Out of the three chambers of the diffusion cell that I ran, only one of them actually…
While Chris and I were in Boston we deposited our membrane stack on a patterned wafer. It was annealed at 1000 C and then etched. Because we didn’t have an accurate deposition rate for Silicon, we overestimated and put down 30 nm (instead of 15 nm). Still, we can see in the TEM that pores…
Wafer 612 was made to mimic wafer 504: 850 C RTP, cool down, 1000 C RTP. Wafer 615 was annealed in two steps: first to 400 C and then to 1000 C (without inbetween cool down). Both wafers showed a lower pinhole density. Wafer 615 had a few “traditional” round pinholes. Wafer 612 had some…
Introduction One area of use to explore for the membranes is desalting, the removal of salts from solutions (such as those containing proteins). Ideal membranes have uniform pore distributions, high mechanical strength, tunable, sharp pore cutoffs, and high porosity. In this study intact samples from W565, which showed no air permeability but allowed rhodamine to…
I am in the process of inspecting each membrane in the newly created wafer 612. First of all, some samples (~10% counted so far) have tears on the membrane edges, shown below. Images were taken at 20x microscope magnification (reflection image followed by transmission). I also have been seeing circular defects which do not penetrate…
Just an update on the PDMS-pncSi closed flow systems progress… The issues we were having early last week with bonding seem to have been solved. We used to run extra ozone flow for ten minutes after the initial 5minutes of UV Ozone. As it turns out this is unnecessary, we are able to get nearly…
I re-ran the rhodamine dilutions narrowing in on the molarities within the range readable by the spectrometer. My start sample was .5mM of Rhodamine. The wavelength I set the spectrometer at was 530nm with 3 trials of each dilution. I started with the least concentrated dilution and cleaned the cuvette between every trial so as…
We’ve built a set up for this but it’s not incredibly user friendly right now. It consists of a camera and a stage. Using the macro function of the camera you can take a close up image of the chip, but you must make sure the horizon line is straight i.e. camera and stage are…
Here is a summary of results for membranes (w510X) that have been annealed without their protective oxide. 800 C RTP @ 10 C/s in susceptor 850 C RTP @ 10 C/s in susceptor 900 C RTP @ 10 C/s in susceptor 950 C RTP @ 10 C/s in susceptor It seems that annealing between 800…
I fixed the graph for the serial dilution of Rhodamine that I did last week and removed all absorbency values that were greater than 1.08 AU. The graph looks much more linear now with an R^2 value of .9517