Last week, I attended the American Society of Exosomes and Microvesicles (ASEMV) conference in Monterey, Ca and presented a poster on the work that I’ve been doing. Despite a very low turnout for my poster session (due to the scheduling of a long break before and dinner after), I met with some very interesting people…
Hi all, This is a “take two” of the mock figure for our manuscript titled “Porous substrates influence early endothelial migration and the associated fibronectin fibrillogenesis”. Many new figures are produced based on the reviewer suggested experiments. It is likely that we will have the word”early” from our manuscript title. PS: The old, raw mock…
For last many months, I am working on neutrophil migration experiments to develop protocols to inhibit them from traversing the basement membrane. My previous blogs are here and here. I want to prevent neutrophils from crossing the endothelial basement membrane (BM), and record the changes in TEER and try to correlate with the gain in…
My goal and focus of the last post is to to use the existing SU-8 grid structure used on the ultrathin silicon oxide lift off membranes to create . To accomplish the goal the SU-8 surface needs to be modied to decrease cell adhesion so that the cells are trapped in the wells. Please see…
This post offers an update on membrane fabrication through nanosphere lithography (NSL), describing in more detail the releasing step as well as the membrane integration into PDMS devices. Membrane lift-off is achieved by dissolving a ZnO film underneath the membrane. After fabrication the sample is immersed in 1M HCl solution. The figure below shows a…
So this is basically an update of my post from a couple of weeks ago (and a figure from the poster that I just put up), but it is a very good status update of the contamination experiments that I have been running with XPS. Basically, I ran the sample that had been purified by…
This will be a very brief post, but kind of an interesting one. I have been attempting to get my identification of exosomes by immunogold labeling and that hasn’t been going terribly well. I think that it is due to a wealth of complications including a lack of fixed exosomes, salt contamination and other problems….
Hey all, I have the first draft of my ASEMV poster here for any feedback that you might have. The conference is in Monterey, CA next week and any feedback that you have on this by Tuesday or Wednesday evening of this week on the poster would be awesome. ASEMV_Poster_KL_09132017_01
What is SiR-DNA? It is a far-red DNA stain, known as SiR-Hoechst, and can be used for live-cell imaging. Most current DNA dyes are toxic or affect the cell’s motion or proliferation. https://www.nature.com/articles/ncomms9497 It does not affect the migration of human dermal fibroblast cells (hdFb). The motility parameters (speed and displacement) for a 24 hour…
Hello Everyone, I have been working with Track Etched membranes since last year and we always had problems with leaking and also not being able to push the flow through the membrane. At the end of the summer, i decided to test my new idea for my devices and finally i have a system which…