NRG

I’ve been trying to recover a pore distribution from my 1085 MgF2 SERS wafer that had a ~60 nm pore size on average. This has been difficult with thinner, 50 nm NPN because there is less material to shape; it’s harder to stop at a certain point to get the right pore distribution. So, using…

Last week we were able to capture and image individual virions on the nano membrane without a PETL using a simple drop-on-membrane protocol to establish some pull through. This was done to to verify that the individual virions will actually be caught in the pores and that our EM preparation protocol was efficient for drying…

One of the issues with my microporous MgF2 cell culture experiments is the apparent multilayer of cells that form on the freestanding area; ideally we would have a nice cell monolayer for imaging. It has been unclear if this was an effect of overseeding or if cells were migrating to the freestanding area. To this…

There are four main challenges for making such a thin photoresist gasket on a little chip. Q1: How can I get under 1um thickness photoresist gasket? Q2: Is the adhesion of photoresist gasket strong enough for further usage? Q3: How can I write the pattern onto the gasket that will have the center window open?…

For many months, Emma and I are trying to achieve neutrophil migration through endothelial cells and through the underlying collagen gel. I need to explain the details of my device first. The schematic is shown as below. I am using a 3 um porous membrane to allow neutrophils to cross the HUVECs in the collagen…

Grand Ballroom A Chair: Baoxia Mi & Zhiping Lai 09:00-09:25 [O8.01] Synthesis of graphene oxide membranes and their behavior in water and isopropanol A. Aher*1, Y. Cai1, M. Majumder2, D. Bhattacharyya1 1University of Kentucky, USA, 2Monash University, Austria 09:25-09:50 [O8.02] Carbon nanotube hollow fiber membranes R. McGinnis*1, K. Reimund2, B. Freeman2, L. Xia3, J. McCutcheon3 1Angstrom…

Last meeting, we discussed the possibility of the storage gel-boxes outgassing a residue onto the porous SiO2 membranes, as evidenced by cells not adhering to the membranes and difficulty in acquiring SEM images. Possible Gel-Box Outgassing on Porous SiO2 Membranes Several cleaning methods were utilized in attempt to clean the membranes, such as O2 plasma,…

Intro For the past couple months, I have been working with Tejas to recreate his previous experiments using a more complex system. The goal of the research is to study the transmigration of neutrophils and represent how they behave during chronic inflammation in vitro. To do so, we planned to use a setup that included…