Hello Everyone, So as you probably remember, we were able to show the capturing and releasing successfully. In addition, we have a Matlab Code now which helps us for intensity change, so as you can see in the next figure which is the analyzed data for videos at the top, it shows capturing gradually…
For the past few weeks, Henry and I have been working with human dermal fibroblast cells and 3T3 mice fibroblast cells. We have been working to quantify wound recovery in hopes to be able to incorporate exosomes from different cell lines to see the effects on wound healing. The NIH 3T3 cells are small cells…
Thanks to UR cleanroom and Jim Mitchell, we finally have a better way to deposit the ZnO film that our fabrication process uses as sacrificial layer. Below is a quick comparison of the previous ZnO deposited by sol-gel and the new ZnO deposited by sputtering at UR. Hopefully, if you look close enough (or far…
Summary Surface roughness is believed to impact the SERS effect. Rougher surfaces tend to produce more SERS activity. We can manipulate the npMgF2 surface by changing the deposition temperature, in the form of changing the grain nucleation, shifting the overall grain size and roughness. I performed TEM tomography and segmentation to pull out distributions of…
Hello Everyone, This is another quick update over our experiments. Exosome Scale Experiments: In all of our experiments, beads are 100nm and pores are 80nm. As you probably remember, in my last post, i talked about hos the membranes are resisstive and they don’t stand these flow rates, so the flow finds another source in…
Summary My analysis of npMgF2 has relied heavily on both tomogram and segmentation processes. The tomogram generation has thus far been sufficient, giving enough detail to our eye to identify different structures, but turning the tomograms into 3d models has been a lot more labor intensive. Using a neural network classifier (Weka, out of a…
Introduction/Motivation Endothelial cell adherence mechanisms are well characterized in literature to date. Endothelial cells, such as HUVECs, interact with their substrate as well as surrounding cells through focal adhesions (network of proteins involved in cell adhesion; vinculin, talin, FAK, etc.), VE-cadherins, tight junctions, and other membrane bound molecules. These molecules work in combination to allow…
I am developing a process for fabricating microporous Parylene Liftoff membranes. I am interested in these as a more flexible substrate for cell culturing. Parylene is relatively strong (compared to say pdms), so it is feasible to work with lifted off pieces of membranes on the order of 1 micron or less in thickness. I list…
Intro Understanding how the Au film coats the inner part of the npMgF2 is essential to understanding how the photonic properties of the npAu/npMgF2 sensor arise. From SEM, we are able to resolve little cones of Au, but unable to see how rough/homogeneous/open the Au layer is. Our hypothesis is that these little cones of…
Many thanks to JK, Dean and Gunner (Webster HS) for compiling this table. Take homes … NPN-O has smaller pore sizes, lower porosity than NPN at the same thickness (50 nm). All materials have slightly asymmetric pores (~ R1/R2 = 0.75). Top-side and bottom side pore distributions are not the same. The original NPN material…