Within the past few weeks we have been working on finalizing an architecture for PETL dialysis that can be cut out, assembled, and disassembled repetitively with precision. Within the past week we were able to replicate the results Kilean achieved back in March with capturing gold particles in the membrane using tangential two-pump flow. 80…
I know this is my third post today, but this is not a data post. It’s more of a PSA. If you want to add beautiful equations into your post (like I did here) then rather than generating a bunch of images and inserting them into the post, you can simply use . is the…
This data is from about a month ago and it something that Jim has been asking me to put up, so here it finally is. When Tejas runs his experiments, he usually leaves me the last mL of blood that he has from his draw in case I want to do anything with it. One…
Last summer, Tejas and I spent a few days beating our heads against the wall because I was trying to develop a mathematical model of particle capture. Specifically, I was trying to understand what amount of transmembrane pressure we would need to hold a particle on (or in, as we are a politically correct, non-discrimination…
Summary I want to imitate the material properties from 1085 as they have given us the best SERS performance to date. We have the best information that we’ve ever had before imitating the nanostructure and nanotopology from the MgF2. Our belief is that getting infilled Au close together in a nanopore dramatically enhances the electric…
Hello Everyone, As you probably remember, We have been working on optimizing Track Etched membranes devices since last semester, these membranes are 80 nm pore size and our beads are 100 nm in. We have been working on the design to make devices work since the first day of these experiments because of different reasons like…
This post is a recap on the nanosphere lithography based membrane patterning process. The results shown here are from a non-optimized run of the process. The image below shows an overview of the fabrication process used. Below you can see optical micrographs of an etch test we performed on a TEOS sample with the…
In previous experiments attempting to culture ADSC on non-porous and porous (0.5 µm, 3.0 µm) SiO2 membranes, we were having difficulty getting the ADSC to attach to the membranes. A majority of the ADSC did not appear well-spread. While in culture or staining, ADSC would detach from the membrane with little to no agitation. Changes…
Hi all, Recently we finished drafting the manuscript titled “Porous substrates promote early endothelial migration at the expense of fibronectin fibrillogenesis.” This manuscript will feature the cover illustrated by Brad (our new medical illustrator in the Gaborski Lab): Long story short (another illustration by Brad): Left (nonporous control substrate): cell traveled slower, covered smaller…
We are happy to be back this summer. Anthony has been working on setting up a system that mimics Kilean’s dialysis devices. Our approach is different in two important ways. First, we are using PETLs, instead of PDMS/glass. For those who are not familiar with PETLs, here is a short summary: Commercial laminating pouches made…